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PHLDA3 antibody [4B6]

Anti-PHLDA3 antibody [4B6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00700
Anti-PHLDA3 antibody [4B6] used in Western Blot (WB). GTX00700

Cat No. GTX00700

Host

Mouse

Clonality

Monoclonal

Clone Name

4B6

Isotype

IgG2b

Application

WB, ICC/IF

Reactivity

Human
Package
100 μg ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1:1000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to N terminal amino acids 1-31 of human PHLDA

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

pleckstrin homology like domain family A member 3 , TIH1

Cellular Localization

Cytoplasm,Membrane

Database

Research Area

DATA IMAGES

Anti-PHLDA3 antibody [4B6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00700

GTX00700 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00700 PHLDA3 antibody [4B6].
Dilution : 1:1000
Fixation : 4% PFA
Permeabilization: 0.25% Triton X-100 in PBS for 10 min

Anti-PHLDA3 antibody [4B6] used in Western Blot (WB). GTX00700

GTX00700 WB Image

WB analysis of MDA-MB-468 cell lysate using GTX00700 PHLDA3 antibody [4B6]. PHLDA3 protein was induced by Ad-p53 in MDA-MB-468 cells (left). PHLDA3 expression was inhibited by shRNA targeting PHLDA3 (right).

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 359