GeneTex
United States (US)

PHLP antibody

GTX107089_WB_19011506_712.jpg
GTX107089_IFA_19011506_374.jpg
GTX107089_39981_IHC_M_19011506_923.jpg
GTX107089_39981_IHC_M_2_19011506_869.jpg

Cat No. GTX107089

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PHLP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp564M1863 antibody, PhLP antibody, PDCL antibody, phosducin-like protein antibody, phosducin-like antibody

Background

Phosducin-like protein is a putative modulator of heterotrimeric G proteins. The protein shares extensive amino acid sequence homology with phosducin, a phosphoprotein expressed in retina and pineal gland. Both phosducin-like protein and phosphoducin have been shown to regulate G-protein signaling by binding to the beta-gamma subunits of G proteins. [provided by RefSeq]

Database

DATA IMAGES
GTX107089_WB_19011506_712.jpg

GTX107089 WB Image

Sample(30 ug whole cell lysate)
A:H1299
10% SDS PAGE
GTX107089 diluted at 1:3000

GTX107089_IFA_19011506_374.jpg

GTX107089 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PHLP(GTX107089) antibody at 1:200 dilution.

GTX107089_39981_IHC_M_19011506_923.jpg

GTX107089 IHC-P Image

PHLP antibody detects PHLP protein at cytoplasm on mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
PHLP antibody (GTX107089) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107089_39981_IHC_M_2_19011506_869.jpg

GTX107089 IHC-P Image

PHLP antibody detects PHLP protein at cytoplasm on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
PHLP antibody (GTX107089) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169