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PHLP antibody

Anti-PHLP antibody used in Western Blot (WB). GTX107089
Anti-PHLP antibody used in IHC (Paraffin sections) (IHC-P). GTX107089
Anti-PHLP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107089
Anti-PHLP antibody used in IHC (Paraffin sections) (IHC-P). GTX107089

Cat. No. GTX107089

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PHLP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PHLP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951136

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosducin like , PhLP

Background

Phosducin-like protein is a putative modulator of heterotrimeric G proteins. The protein shares extensive amino acid sequence homology with phosducin, a phosphoprotein expressed in retina and pineal gland. Both phosducin-like protein and phosphoducin have been shown to regulate G-protein signaling by binding to the beta-gamma subunits of G proteins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PHLP antibody used in Western Blot (WB). GTX107089

GTX107089 WB Image

Sample(30 ug whole cell lysate)
A:H1299
10% SDS PAGE
GTX107089 diluted at 1:3000

Anti-PHLP antibody used in IHC (Paraffin sections) (IHC-P). GTX107089

GTX107089 IHC-P Image

PHLP antibody detects PHLP protein at cytoplasm on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
PHLP antibody (GTX107089) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PHLP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107089

GTX107089 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PHLP(GTX107089) antibody at 1:200 dilution.

Anti-PHLP antibody used in IHC (Paraffin sections) (IHC-P). GTX107089

GTX107089 IHC-P Image

PHLP antibody detects PHLP protein at cytoplasm on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
PHLP antibody (GTX107089) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169