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PHLPP1 antibody, N-term

Anti-PHLPP1 antibody, N-term used in Western Blot (WB). GTX81275

Cat. No. GTX81275

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
400 μl ($399)
See all PHLPP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
Not tested in other applications.

Calculated MW

185 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 531-560 amino acids from the N-terminal region of human PHLPP1.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PH domain and leucine rich repeat protein phosphatase 1 , PHLPP , PLEKHE1 , PPM3A , SCOP

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the serine/threonine phosphatase family. The encoded protein promotes apoptosis by dephosphorylating and inactivating the serine/threonine kinase Akt, and functions as a tumor suppressor in multiple types of cancer. Increased expression of this gene may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling. [provided by RefSeq, Dec 2011]

Database

Research Area

DATA IMAGES

Anti-PHLPP1 antibody, N-term used in Western Blot (WB). GTX81275

GTX81275 WB Image

WB analysis of K562 whole cell lysate using GTX81275 PHLPP1 antibody, N-term.
Loading : 20 μg per lane
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399