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PHLPP1 antibody

Anti-PHLPP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31861
Anti-PHLPP1 antibody used in Western Blot (WB). GTX31861
Anti-PHLPP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31861

Cat No. GTX31861

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA
Reactivity Human
Package
100 μg ($339)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

185 kDa. ( Note )

Positive Control

SW480 Cell Lysate(GTX23957)

Specificity/Sensitivity

At least four isoforms are known to exist; this antibody will only detect the largest isoform. PHLPP1 antibody is predicted to not cross react with PHLPP2.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PHLPP1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PHLPP1.The immunogen is located within amino acids 250 - 300 of PHLPP1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ph Domain And Leucine Rich Repeat Protein Phosphatase 1,Phlpp,Plekhe1,Ppm3A,Scop,Phlpp1

Background

This gene encodes a member of the serine/threonine phosphatase family. The encoded protein promotes apoptosis by dephosphorylating and inactivating the serine/threonine kinase Akt, and functions as a tumor suppressor in multiple types of cancer. Increased expression of this gene may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling. [provided by RefSeq, Dec 2011]

Database

Research Area

DATA IMAGES
Anti-PHLPP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31861

GTX31861 ICC/IF Image

ICC/IF analysis of SW480 cells using GTX31861 PHLPP1 antibody.
Working concentration : 5 μg/ml

Anti-PHLPP1 antibody used in Western Blot (WB). GTX31861

GTX31861 WB Image

WB analysis of SW480 cell lysate using GTX31861 PHLPP1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-PHLPP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31861

GTX31861 ICC/IF Image

ICC/IF analysis of SW480 cells using GTX31861 PHLPP1 antibody.
Working concentration : 2.5 μg/ml

REFERENCE
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REVIEW
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Package List Price ($)
$ 339