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PHLPP1 antibody

Anti-PHLPP1 antibody used in Western Blot (WB). GTX31861
Anti-PHLPP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31861
Anti-PHLPP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31861

Cat. No. GTX31861

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all PHLPP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

185 kDa. ( Note )

Positive Control

SW480 Cell Lysate(GTX23957)

Product Note

At least four isoforms are known to exist; this antibody will only detect the largest isoform. PHLPP1 antibody is predicted to not cross react with PHLPP2.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PHLPP1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PHLPP1.The immunogen is located within amino acids 250 - 300 of PHLPP1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PH domain and leucine rich repeat protein phosphatase 1 , PHLPP , PLEKHE1 , PPM3A , SCOP

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the serine/threonine phosphatase family. The encoded protein promotes apoptosis by dephosphorylating and inactivating the serine/threonine kinase Akt, and functions as a tumor suppressor in multiple types of cancer. Increased expression of this gene may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling. [provided by RefSeq, Dec 2011]

Database

Research Area

DATA IMAGES

Anti-PHLPP1 antibody used in Western Blot (WB). GTX31861

GTX31861 WB Image

WB analysis of SW480 cell lysate using GTX31861 PHLPP1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-PHLPP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31861

GTX31861 ICC/IF Image

ICC/IF analysis of SW480 cells using GTX31861 PHLPP1 antibody.
Working concentration : 2.5 μg/ml

Anti-PHLPP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31861

GTX31861 ICC/IF Image

ICC/IF analysis of SW480 cells using GTX31861 PHLPP1 antibody.
Working concentration : 5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399