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PHOX2B antibody

Anti-PHOX2B antibody used in Western Blot (WB). GTX109677
Anti-PHOX2B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109677

Cat. No. GTX109677

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PHOX2B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

HeLaS3

Predict Reactivity

Mouse, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human PHOX2B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951223

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

paired like homeobox 2B , CCHS , NBLST2 , NBPhox , PMX2B

Cellular Localization

Nucleus

Background

The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription factor involved in the development of several major noradrenergic neuron populations and the determination of neurotransmitter phenotype. The gene product is linked to enhancement of second messenger-mediated activation of the dopamine beta-hydroylase, c-fos promoters and several enhancers, including cyclic amp-response element and serum-response element. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PHOX2B antibody used in Western Blot (WB). GTX109677

GTX109677 WB Image

Sample(30 ug whole cell lysate)
A:HeLa S3(GTX14654)
12% SDS PAGE
GTX109677 diluted at 1:1000

Anti-PHOX2B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109677

GTX109677 ICC/IF Image

PHOX2B antibody detects PHOX2B protein at nucleus by immunofluorescent analysis.
Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PHOX2B protein stained by PHOX2B antibody (GTX109677) diluted at 1:1000.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Ritchie Ho et al. Cell Syst 2021; 12 (2) : 159-175.e9 Cross-Comparison of Human iPSC Motor Neuron Models of Familial and Sporadic ALS Reveals Early and Convergent Transcriptomic Disease Signatures.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169