APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 5μg/ml |
|
Note :
IHC-P
Human Placenta shows cytoplasm staining of cytotrophoblasts and mesenchymal cells.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89716-PEP).
Predict Reactivity
Mouse, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-TVKWYQLTAATYL, from the C Terminus of the protein sequence according to NP_002636.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha , CPK , PI3-K-C2(ALPHA) , PI3-K-C2A , PI3K-C2-alpha , PI3K-C2alpha
Cellular Localization
Cell membrane , Golgi apparatus , Nucleus , Cytoplasm
Background
The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX89716 IHC-P Image
IHC-P analysis of human heart using GTX89716 PI3 kinase C2 alpha antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
|
|
|
GTX89716 IHC-P Image
IHC-P analysis of human placenta using GTX89716 PI3 kinase C2 alpha antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2μg/ml
|
REFERENCE
There are currently no references for PI3 kinase C2 alpha antibody, C-term (GTX89716). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PI3 kinase C2 alpha antibody, C-term (GTX89716). Be the first to share your experience with this product.
