GeneTex
United States (US)

PI3 kinase C2 alpha antibody [C3], C-term

GTX104640_WB_19011506_220.jpg
GTX104640_39890_IFA_19011506_557.jpg
GTX104640_39890_IHC_19011506_207.jpg

Cat No. GTX104640

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

191 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PI3K C2 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phosphatidylinositol-4-Phosphate 3-Kinase Catalytic Subunit Type 2 Alpha,Cpk,Pi3-K-C2(Alpha),Pi3-K-C2A,Pi3K-C2-Alpha,Pi3K-C2Alpha,Pik3C2A

Cellular Localization

Cell membrane , Golgi apparatus , Cytoplasmic vesicle , clathrin-coated vesicle , Nucleus , Cytoplasm

Background

The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104640_WB_19011506_220.jpg

GTX104640 WB Image

Sample (30 µg of whole cell lysate)
A: A431 (GTX27909)
5% SDS PAGE
GTX104640 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104640_39890_IFA_19011506_557.jpg

GTX104640 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using PI3K C2 alpha(GTX104640) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

GTX104640_39890_IHC_19011506_207.jpg

GTX104640 IHC-P Image

PI3K C2 alpha antibody [C3], C-term detects PIK3C2A protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
PI3K C2 alpha antibody [C3], C-term (GTX104640) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169