GeneTex

  • Country / Location Selection

United States (US)

PI3 kinase C2 alpha antibody [HL1612]

Recombinant antibody
Anti-PI3 kinase C2 alpha antibody [HL1612] used in IHC (Paraffin sections) (IHC-P). GTX637103
Anti-PI3 kinase C2 alpha antibody [HL1612] used in Western Blot (WB). GTX637103
Anti-PI3 kinase C2 alpha antibody [HL1612] used in Western Blot (WB). GTX637103
Anti-PI3 kinase C2 alpha antibody [HL1612] used in Western Blot (WB). GTX637103
Anti-PI3 kinase C2 alpha antibody [HL1612] used in Western Blot (WB). GTX637103

Cat. No. GTX637103

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1612

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all PI3 kinase C2 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

191 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human PI3 kinase C2 alpha. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha , CPK , PI3-K-C2(ALPHA) , PI3-K-C2A , PI3K-C2-alpha , PI3K-C2alpha

Cellular Localization

Cell membrane,Golgi apparatus,Nucleus,Cytoplasm

Background

The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PI3 kinase C2 alpha antibody [HL1612] used in IHC (Paraffin sections) (IHC-P). GTX637103

GTX637103 IHC-P Image

PI3 kinase C2 alpha antibody [HL1612] detects PI3 kinase C2 alpha protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
PI3 kinase C2 alpha stained by PI3 kinase C2 alpha antibody [HL1612] (GTX637103) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PI3 kinase C2 alpha antibody [HL1612] used in Western Blot (WB). GTX637103

GTX637103 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase C2 alpha antibody [HL1612] (GTX637103) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PI3 kinase C2 alpha antibody [HL1612] used in Western Blot (WB). GTX637103

GTX637103 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase C2 alpha antibody [HL1612] (GTX637103) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-PI3 kinase C2 alpha antibody [HL1612] used in Western Blot (WB). GTX637103

GTX637103 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase C2 alpha antibody [HL1612] (GTX637103) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PI3 kinase C2 alpha antibody [HL1612] used in Western Blot (WB). GTX637103

GTX637103 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase C2 alpha antibody [HL1612] (GTX637103) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

There are currently no references for PI3 kinase C2 alpha antibody [HL1612] (GTX637103). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for PI3 kinase C2 alpha antibody [HL1612] (GTX637103). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399
$ 169
Custom Conjugations