GeneTex
United States (US)

PI3 kinase p110 alpha antibody

GTX100462_40254_IHC_19011506_342.jpg
GTX100462_42585_20160907_IFA_19011506_159.jpg
GTX100462_42585_20180727_WB_19011506_959.jpg
GTX100462_41605_20160929_WB_M_brain_19011506_634.jpg
GTX100462_40254_WB_M_19011506_120.jpg

Cat No. GTX100462

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

Positive Control

H1299 , HCT116 , C2C12 , C2C12+AEA10mM , mouse brain

Predict Reactivity

Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.98mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PI3K p110 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC142161 antibody, MGC142163 antibody, PI3K antibody, p110-alpha antibody, PIK3CA antibody, serine/threonine protein kinase PIK3CA antibody, "phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha antibody", ptdIns-3-kinase subunit p110-alpha antibody, PI3-kinase subunit alpha antibody, PI3Kalpha antibody, PtdIns-3-kinase p110 antibody, "phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform antibody", phosphoinositide-3-kinase catalytic alpha polypeptide antibody, "phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha antibody", PI3-kinase p110 subunit alpha antibody, PI3K-alpha antibody, "phosphatidylinositol 3-kinase, catalytic, alpha polypeptide antibody", p110alpha antibody, "phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform antibody", ptdIns-3-kinase subunit alpha antibody, "phosphoinositide-3-kinase, catalytic, alpha polypeptide antibody"

Background

Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100462_40254_IHC_19011506_342.jpg

GTX100462 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Human Breast Cancer, using PI3 kinase p110 alpha(GTX100462) antibody.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100462_42585_20160907_IFA_19011506_159.jpg

GTX100462 ICC/IF Image

PI3 kinase p110 alpha antibody detects PI3 kinase p110 alpha protein at cytoplasm by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PI3 kinase p110 alpha protein stained by PI3 kinase p110 alpha antibody (GTX100462) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX100462_42585_20180727_WB_19011506_959.jpg

GTX100462 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 alpha antibody (GTX100462) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100462_41605_20160929_WB_M_brain_19011506_634.jpg

GTX100462 WB Image

Mouse tissue extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 alpha antibody (GTX100462) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100462_40254_WB_M_19011506_120.jpg

GTX100462 WB Image

Sample (50 µg of whole cell lysate)
A: C2C12
B: C2C12+AEA10mM
GTX100462 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169