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PI3 kinase p110 alpha antibody

Anti-PI3 kinase p110 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55747
Anti-PI3 kinase p110 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX55747
Anti-PI3 kinase p110 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55747
Anti-PI3 kinase p110 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX55747
Anti-PI3 kinase p110 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX55747
Anti-PI3 kinase p110 alpha antibody used in Western Blot (WB). GTX55747

Cat. No. GTX55747

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all PI3 kinase p110 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 401-600 of human PIK3CA (NP_006209.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha , CLAPO , CLOVE , CWS5 , MCAP , MCM , MCMTC , PI3K , PI3K-alpha , p110-alpha

Background

Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22. [provided by RefSeq, Apr 2016]

Database

Research Area

DATA IMAGES

Anti-PI3 kinase p110 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55747

GTX55747 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX55747 PI3 kinase p110 alpha antibody.
Blue : DAPI
Dilution : 1:100

Anti-PI3 kinase p110 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX55747

GTX55747 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX55747 PI3 kinase p110 alpha antibody.
Dilution : 1:100

Anti-PI3 kinase p110 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55747

GTX55747 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX55747 PI3 kinase p110 alpha antibody.
Blue : DAPI
Dilution : 1:100

Anti-PI3 kinase p110 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX55747

GTX55747 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX55747 PI3 kinase p110 alpha antibody.
Dilution : 1:100

Anti-PI3 kinase p110 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX55747

GTX55747 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX55747 PI3 kinase p110 alpha antibody.
Dilution : 1:100

Anti-PI3 kinase p110 alpha antibody used in Western Blot (WB). GTX55747

GTX55747 WB Image

WB analysis of HeLa cell lysate using GTX55747 PI3 kinase p110 alpha antibody.
Dilution : 1:500
Loading : 25μg per lane

REFERENCE

Application Reference

Lu R et al. Evid Based Complement Alternat Med 2022; 2022 : 4838650 Network Pharmacology and Experimental Validation to Reveal Effects and Mechanisms of Icariin Combined with Nobiletin against Chronic Obstructive Pulmonary Diseases.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399