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PI3 kinase p110 beta antibody [N3C2], Internal

GTX111173_WB_19011507_927.jpg
GTX111173_40073_20160629_IHC-P_R_19011507_836.jpg
GTX111173_40073_WB_M_19011507_780.jpg
GTX111173_40073_WB_R_19011507_470.jpg
GTX111173_40073_IFA_19011507_855.jpg

Cat No. GTX111173

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

123 kDa. ( Note )

Positive Control

H1299 , C2C12 , C2C12+AEA10mM , PC-12

Predict Reactivity

Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PI3K p110 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp779K1237 antibody, MGC133043 antibody, P110BETA antibody, PI3K antibody, PI3KBETA antibody, PIK3C1 antibody, PIK3CB antibody, ptdIns-3-kinase subunit beta antibody, PtdIns-3-kinase p110 antibody, PI3K-beta antibody, PI3-kinase p110 subunit beta antibody, PI3-kinase subunit beta antibody, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform antibody, phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta antibody, ptdIns-3-kinase subunit p110-beta antibody, phosphoinositide-3-kinase, catalytic, beta polypeptide antibody

Background

Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. They have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kD catalytic subunit, such as PIK3CB, and an 85-kD adaptor subunit (Hu et al., 1993 [PubMed 8246984]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX111173_WB_19011507_927.jpg

GTX111173 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX111173 diluted at 1:1000

GTX111173_40073_20160629_IHC-P_R_19011507_836.jpg

GTX111173 IHC-P Image

PI3K p110 beta antibody [N3C2], Internal detects PI3K p110 beta protein at cytoplasm and nucleus in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
PI3K p110 beta antibody [N3C2], Internal (GTX111173) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX111173_40073_WB_M_19011507_780.jpg

GTX111173 WB Image

Sample (50 ug of whole cell lysate)
A: C2C12
B: C2C12+AEA10mM
GTX111173 diluted at 1:1000

GTX111173_40073_WB_R_19011507_470.jpg

GTX111173 WB Image

PI3K p110 beta antibody [N3C2], Internal detects PIK3CB protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
5 % SDS-PAGE
PI3K p110 beta antibody [N3C2], Internal (GTX111173) dilution: 1:1000

GTX111173_40073_IFA_19011507_855.jpg

GTX111173 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PI3K p110 beta(GTX111173) antibody at 1:200 dilution.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf