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PI3 kinase p110 beta antibody [N3C2], Internal

Anti-PI3 kinase p110 beta antibody [N3C2], Internal used in Western Blot (WB). GTX111173
Anti-PI3 kinase p110 beta antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111173
Anti-PI3 kinase p110 beta antibody [N3C2], Internal used in Western Blot (WB). GTX111173
Anti-PI3 kinase p110 beta antibody [N3C2], Internal used in Western Blot (WB). GTX111173
Anti-PI3 kinase p110 beta antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111173

Cat No. GTX111173

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

123 kDa. ( Note )

Positive Control

H1299 , C2C12 , C2C12+AEA10mM , PC-12

Predict Reactivity

Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PI3K p110 beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phosphatidylinositol-4,5-Bisphosphate 3-Kinase Catalytic Subunit Beta,P110Beta,Pi3K,Pi3Kbeta,Pik3C1,Pik3Cb

Background

Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. They have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kD catalytic subunit, such as PIK3CB, and an 85-kD adaptor subunit (Hu et al., 1993 [PubMed 8246984]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-PI3 kinase p110 beta antibody [N3C2], Internal used in Western Blot (WB). GTX111173

GTX111173 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX111173 diluted at 1:1000

Anti-PI3 kinase p110 beta antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111173

GTX111173 IHC-P Image

PI3K p110 beta antibody [N3C2], Internal detects PI3K p110 beta protein at cytoplasm and nucleus in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
PI3K p110 beta antibody [N3C2], Internal (GTX111173) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PI3 kinase p110 beta antibody [N3C2], Internal used in Western Blot (WB). GTX111173

GTX111173 WB Image

Sample (50 ug of whole cell lysate)
A: C2C12
B: C2C12+AEA10mM
GTX111173 diluted at 1:1000

Anti-PI3 kinase p110 beta antibody [N3C2], Internal used in Western Blot (WB). GTX111173

GTX111173 WB Image

PI3K p110 beta antibody [N3C2], Internal detects PIK3CB protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
5 % SDS-PAGE
PI3K p110 beta antibody [N3C2], Internal (GTX111173) dilution: 1:1000

Anti-PI3 kinase p110 beta antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111173

GTX111173 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PI3K p110 beta(GTX111173) antibody at 1:200 dilution.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169