GeneTex
United States (US)

PI3 kinase p110 gamma antibody [C3], C-term

GTX104639_39631_20160428_WB_R_19011506_545.jpg
GTX104639_39631_20180112_WB_B_19011506_631.jpg
GTX104639_39631_20180112_WB_M_19011506_399.jpg
GTX104639_39631_IHC_19011506_869.jpg

Cat No. GTX104639

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

126 kDa. ( Note )

Positive Control

PIK3CG-transfected 293T , Neuro 2A , Raw264.7 , C8D30 , PC-12

Predict Reactivity

Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PI3K p110 gamma. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

1-phosphatidylinositol 3-kinase antibody, p110-gamma antibody, p120-PI3K antibody, phosphatidylinositol 3 kinase gamma, p110 gamma antibody, phosphatidylinositol 3-kinase catalytic 110-kD gamma antibody, phosphatidylinositol 3-kinase, catalytic, gamma polypeptide antibody, phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma antibody, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma isoform antibody, phosphoinositide-3-kinase gamma catalytic subunit antibody, phosphoinositide-3-kinase, catalytic, gamma polypeptide antibody, PI3-kinase subunit gamma antibody, PI3CG antibody, PI3K antibody, PI3K-gamma antibody, PIK3 antibody, PIK3CG antibody, ptdIns-3-kinase subunit gamma antibody, ptdIns-3-kinase subunit p110-gamma antibody, serine/threonine protein kinase PIK3CG antibody

Background

Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I catalytic subunit of PI3K. Like other class I catalytic subunits (p110-alpha p110-beta, and p110-delta), the encoded protein binds a p85 regulatory subunit to form PI3K. This gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jun 2015]

Database

Research Area

DATA IMAGES
GTX104639_39631_20160428_WB_R_19011506_545.jpg

GTX104639 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 gamma antibody [C3], C-term (GTX104639) diluted at 1:1000.

GTX104639_39631_20180112_WB_B_19011506_631.jpg

GTX104639 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 gamma antibody [C3], C-term (GTX104639) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104639_39631_20180112_WB_M_19011506_399.jpg

GTX104639 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 gamma antibody [C3], C-term (GTX104639) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104639_39631_IHC_19011506_869.jpg

GTX104639 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Human Breast Cancer, using PI3K gamma(GTX104639) antibody.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf