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PI3 kinase p110 gamma antibody [C3], C-term

Anti-PI3 kinase p110 gamma antibody [C3], C-term used in Western Blot (WB). GTX104639
Anti-PI3 kinase p110 gamma antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104639
Anti-PI3 kinase p110 gamma antibody [C3], C-term used in Western Blot (WB). GTX104639
Anti-PI3 kinase p110 gamma antibody [C3], C-term used in Western Blot (WB). GTX104639

Cat. No. GTX104639

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PI3 kinase p110 gamma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

126 kDa. ( Note )

Positive Control

Neuro 2A , Raw264.7 , C8D30 , PIK3CG-transfected 293T

Predict Reactivity

Zebrafish, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PI3 kinase p110 gamma. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241185

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma , PI3CG , PI3K , PI3Kgamma , PIK3 , p110gamma , p120-PI3K

Background

Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I catalytic subunit of PI3K. Like other class I catalytic subunits (p110-alpha p110-beta, and p110-delta), the encoded protein binds a p85 regulatory subunit to form PI3K. This gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jun 2015]

Database

Research Area

DATA IMAGES

Anti-PI3 kinase p110 gamma antibody [C3], C-term used in Western Blot (WB). GTX104639

GTX104639 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 gamma antibody [C3], C-term (GTX104639) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PI3 kinase p110 gamma antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104639

GTX104639 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Human Breast Cancer, using PI3K gamma(GTX104639) antibody.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PI3 kinase p110 gamma antibody [C3], C-term used in Western Blot (WB). GTX104639

GTX104639 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 gamma antibody [C3], C-term (GTX104639) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PI3 kinase p110 gamma antibody [C3], C-term used in Western Blot (WB). GTX104639

GTX104639 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 gamma antibody [C3], C-term (GTX104639) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169