GeneTex
United States (US)

PI3 kinase p150 antibody [C1C3]

KO/KD
GTX108953_40016_20161006_WB_shRNA_watermark_19011507_463.jpg
GTX108953_WB_19011507_856.jpg
GTX108953_40016_20150209_IP_19011507_173.jpg

Cat No. GTX108953

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

153 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PI3-Kinase p150. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC102700 antibody, VPS15 antibody, p150 antibody, PIK3R4 antibody, PI3-kinase p150 subunit antibody, "phosphoinositide-3-kinase, regulatory subunit 4, p150 antibody", phosphoinositide 3-kinase adaptor protein antibody, phosphatidylinositol 3-kinase-associated p150 antibody, PI3-kinase regulatory subunit 4 antibody, phosphoinositide 3-kinase regulatory subunit 4 antibody, "phosphoinositide-3-kinase, regulatory subunit 4 antibody"

Background

Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway.

Database

DATA IMAGES
GTX108953_40016_20161006_WB_shRNA_watermark_19011507_463.jpg

GTX108953 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody [C1C3] (GTX108953) diluted at 1:500.

GTX108953_WB_19011507_856.jpg

GTX108953 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
B: Raji
7.5% SDS PAGE
GTX108953 diluted at 1:1000

GTX108953_40016_20150209_IP_19011507_173.jpg

GTX108953 IP Image

Immunoprecipitation of PI3-Kinase p150 protein from HeLa whole cell extracts using 5 μg of PI3-Kinase p150 antibody [C1C3] (GTX108953).
Western blot analysis was performed using PI3-Kinase p150 antibody [C1C3] (GTX108953).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169