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PI3 kinase p150 antibody [C1C3]

Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953
Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953
Anti-PI3 kinase p150 antibody [C1C3] used in Immunoprecipitation (IP). GTX108953

Cat No. GTX108953

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

153 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PI3-Kinase p150. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

phosphoinositide-3-kinase regulatory subunit 4,VPS15,p150

Background

Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway.

Database

DATA IMAGES
Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953

GTX108953 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody [C1C3] (GTX108953) diluted at 1:500.

Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953

GTX108953 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
B: Raji
7.5% SDS PAGE
GTX108953 diluted at 1:1000

Anti-PI3 kinase p150 antibody [C1C3] used in Immunoprecipitation (IP). GTX108953

GTX108953 IP Image

Immunoprecipitation of PI3-Kinase p150 protein from HeLa whole cell extracts using 5 μg of PI3-Kinase p150 antibody [C1C3] (GTX108953).
Western blot analysis was performed using PI3-Kinase p150 antibody [C1C3] (GTX108953).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169