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PI3 kinase p150 antibody

Anti-PI3 kinase p150 antibody used in Western Blot (WB). GTX132466
Anti-PI3 kinase p150 antibody used in IHC (Paraffin sections) (IHC-P). GTX132466
Anti-PI3 kinase p150 antibody used in Western Blot (WB). GTX132466
Anti-PI3 kinase p150 antibody used in Immunoprecipitation (IP). GTX132466
Anti-PI3 kinase p150 antibody used in Western Blot (WB). GTX132466
Anti-PI3 kinase p150 antibody used in Western Blot (WB). GTX132466
Anti-PI3 kinase p150 antibody used in Western Blot (WB). GTX132466

Cat No. GTX132466

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

153 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Jurkat , Raji , NCI-H929 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , mouse liver

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

4.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PI3-Kinase p150. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

phosphoinositide-3-kinase regulatory subunit 4,VPS15,p150

Background

Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway.

Database

DATA IMAGES
Anti-PI3 kinase p150 antibody used in Western Blot (WB). GTX132466

GTX132466 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody (GTX132466) diluted at 1:1000.

Anti-PI3 kinase p150 antibody used in IHC (Paraffin sections) (IHC-P). GTX132466

GTX132466 IHC-P Image

PI3-Kinase p150 antibody detects PI3-Kinase p150 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
PI3-Kinase p150 antibody (GTX132466) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PI3 kinase p150 antibody used in Western Blot (WB). GTX132466

GTX132466 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody (GTX132466) diluted at 1:1000.

Anti-PI3 kinase p150 antibody used in Immunoprecipitation (IP). GTX132466

GTX132466 IP Image

Immunoprecipitation of PI3-Kinase p150 protein from HeLa whole cell extracts using 5 μg of PI3-Kinase p150 antibody (GTX132466).
Western blot analysis was performed using PI3-Kinase p150 antibody (GTX132466).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-PI3 kinase p150 antibody used in Western Blot (WB). GTX132466

GTX132466 WB Image

Mouse tissue extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody (GTX132466) diluted at 1:1000.

Anti-PI3 kinase p150 antibody used in Western Blot (WB). GTX132466

GTX132466 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody (GTX132466) diluted at 1:1000.

Anti-PI3 kinase p150 antibody used in Western Blot (WB). GTX132466

GTX132466 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody (GTX132466) diluted at 1:1000.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169