APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat , Raji , NCI-H929 , 293T , A431 , HeLa , HepG2 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , mouse liver
Predict Reactivity
Rat, Bovine, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
4.61 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human PI3 kinase p150. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886653
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
phosphoinositide-3-kinase regulatory subunit 4 , VPS15 , p150
Background
Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway.
Database
Research Area
DATA IMAGES
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GTX132466 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody (GTX132466) diluted at 1:1000.
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GTX132466 IHC-P Image
PI3-Kinase p150 antibody detects PI3-Kinase p150 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
PI3-Kinase p150 antibody (GTX132466) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX132466 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody (GTX132466) diluted at 1:1000.
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GTX132466 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody (GTX132466) diluted at 1:1000.
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GTX132466 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody (GTX132466) diluted at 1:1000.
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GTX132466 WB Image
Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody (GTX132466) diluted at 1:1000.
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GTX132466 IP Image
Immunoprecipitation of PI3-Kinase p150 protein from HeLa whole cell extracts using 5 μg of PI3-Kinase p150 antibody (GTX132466).
Western blot analysis was performed using PI3-Kinase p150 antibody (GTX132466) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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REFERENCE
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REVIEW
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