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PI3 kinase p85 alpha antibody [6G10]

Anti-PI3 kinase p85 alpha antibody [6G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60633
Anti-PI3 kinase p85 alpha antibody [6G10] used in Flow cytometry (FACS). GTX60633
Anti-PI3 kinase p85 alpha antibody [6G10] used in Western Blot (WB). GTX60633
Anti-PI3 kinase p85 alpha antibody [6G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60633
Anti-PI3 kinase p85 alpha antibody [6G10] used in IHC (Paraffin sections) (IHC-P). GTX60633
Anti-PI3 kinase p85 alpha antibody [6G10] used in IHC (Paraffin sections) (IHC-P). GTX60633
Anti-PI3 kinase p85 alpha antibody [6G10] used in ELISA (ELISA). GTX60633

Cat. No. GTX60633

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6G10

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($409)
See all PI3 kinase p85 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/100
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human PI3 kinase p85 alpha (AA: 159-388) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

RRID

AB_2887991

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoinositide-3-kinase regulatory subunit 1 , AGM7 , GRB1 , IMD36 , p85 , p85-ALPHA

Background

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

Database

Research Area

DATA IMAGES

Anti-PI3 kinase p85 alpha antibody [6G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60633

GTX60633 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60633 PI3 kinase p85 alpha antibody [6G10].
Green : PI3 kinase p85 alpha
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-PI3 kinase p85 alpha antibody [6G10] used in Flow cytometry (FACS). GTX60633

GTX60633 FACS Image

FACS analysis of NIH3T3 cells using GTX60633 PI3 kinase p85 alpha antibody [6G10].
Green : PI3 kinase p85 alpha
Red : negative control

Anti-PI3 kinase p85 alpha antibody [6G10] used in Western Blot (WB). GTX60633

GTX60633 WB Image

WB analysis of human PI3 kinase p85 alpha recombinant protein using GTX60633 PI3 kinase p85 alpha antibody [6G10].

Anti-PI3 kinase p85 alpha antibody [6G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60633

GTX60633 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60633 PI3 kinase p85 alpha antibody [6G10].
Green : PI3 kinase p85 alpha
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-PI3 kinase p85 alpha antibody [6G10] used in IHC (Paraffin sections) (IHC-P). GTX60633

GTX60633 IHC-P Image

IHC-P analysis of rectum cancer tissue using GTX60633 PI3 kinase p85 alpha antibody [6G10].

Anti-PI3 kinase p85 alpha antibody [6G10] used in IHC (Paraffin sections) (IHC-P). GTX60633

GTX60633 IHC-P Image

IHC-P analysis of cervical cancer tissue using GTX60633 PI3 kinase p85 alpha antibody [6G10].

Anti-PI3 kinase p85 alpha antibody [6G10] used in ELISA (ELISA). GTX60633

GTX60633 ELISA Image

ELISA analysis of antigen using GTX60633 PI3 kinase p85 alpha antibody [6G10].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

REFERENCE

Application Reference

Piva MBR et al. Cancers (Basel) 2017; 9 (9) integrin activation contributes to lower * sensitivity in mv3 melanoma cells by inducing the wnt signalling pathway.
Application : FACS
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409