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PI3 kinase p85 alpha antibody [HL1398]

Recombinant antibody KO/KD
Anti-PI3 kinase p85 alpha antibody [HL1398] used in Western Blot (WB). GTX636838
Anti-PI3 kinase p85 alpha antibody [HL1398] used in Western Blot (WB). GTX636838
Anti-PI3 kinase p85 alpha antibody [HL1398] used in Western Blot (WB). GTX636838
Anti-PI3 kinase p85 alpha antibody [HL1398] used in Western Blot (WB). GTX636838
Anti-PI3 kinase p85 alpha antibody [HL1398] used in IHC (Paraffin sections) (IHC-P). GTX636838
Anti-PI3 kinase p85 alpha antibody [HL1398] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636838

Cat. No. GTX636838

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1398

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PI3 kinase p85 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PI3 kinase p85 alpha. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910084

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoinositide-3-kinase regulatory subunit 1 , AGM7 , GRB1 , IMD36 , p85 , p85-ALPHA

Background

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

Database

Research Area

DATA IMAGES

Anti-PI3 kinase p85 alpha antibody [HL1398] used in Western Blot (WB). GTX636838

GTX636838 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [HL1398] (GTX636838) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PI3 kinase p85 alpha antibody [HL1398] used in Western Blot (WB). GTX636838

GTX636838 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [HL1398] (GTX636838) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PI3 kinase p85 alpha antibody [HL1398] used in Western Blot (WB). GTX636838

GTX636838 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [HL1398] (GTX636838) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PI3 kinase p85 alpha antibody [HL1398] used in Western Blot (WB). GTX636838

GTX636838 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [HL1398] (GTX636838) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PI3 kinase p85 alpha antibody [HL1398] used in IHC (Paraffin sections) (IHC-P). GTX636838

GTX636838 IHC-P Image

PI3 kinase p85 alpha antibody [HL1398] detects PI3 kinase p85 alpha protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
PI3 kinase p85 alpha stained by PI3 kinase p85 alpha antibody [HL1398] (GTX636838) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PI3 kinase p85 alpha antibody [HL1398] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636838

GTX636838 ICC/IF Image

PI3 kinase p85 alpha antibody [HL1398] detects PI3 kinase p85 alpha protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PI3 kinase p85 alpha stained by PI3 kinase p85 alpha antibody [HL1398] (GTX636838) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations