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PI3 kinase p85 alpha antibody [HL1398]

Anti-PI3 kinase p85 alpha antibody [HL1398] used in Western Blot (WB). GTX636838
Anti-PI3 kinase p85 alpha antibody [HL1398] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636838
Anti-PI3 kinase p85 alpha antibody [HL1398] used in IHC (Paraffin sections) (IHC-P). GTX636838
Anti-PI3 kinase p85 alpha antibody [HL1398] used in Western Blot (WB). GTX636838
Anti-PI3 kinase p85 alpha antibody [HL1398] used in Western Blot (WB). GTX636838
Anti-PI3 kinase p85 alpha antibody [HL1398] used in Western Blot (WB). GTX636838

Cat No. GTX636838

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1398

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PI3 kinase p85 alpha. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910084

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

phosphoinositide-3-kinase regulatory subunit 1 , AGM7 , GRB1 , IMD36 , p85 , p85-ALPHA

Background

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

Database

Research Area

DATA IMAGES

Anti-PI3 kinase p85 alpha antibody [HL1398] used in Western Blot (WB). GTX636838

GTX636838 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [HL1398] (GTX636838) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PI3 kinase p85 alpha antibody [HL1398] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636838

GTX636838 ICC/IF Image

PI3 kinase p85 alpha antibody [HL1398] detects PI3 kinase p85 alpha protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PI3 kinase p85 alpha stained by PI3 kinase p85 alpha antibody [HL1398] (GTX636838) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PI3 kinase p85 alpha antibody [HL1398] used in IHC (Paraffin sections) (IHC-P). GTX636838

GTX636838 IHC-P Image

PI3 kinase p85 alpha antibody [HL1398] detects PI3 kinase p85 alpha protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
PI3 kinase p85 alpha stained by PI3 kinase p85 alpha antibody [HL1398] (GTX636838) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PI3 kinase p85 alpha antibody [HL1398] used in Western Blot (WB). GTX636838

GTX636838 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [HL1398] (GTX636838) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PI3 kinase p85 alpha antibody [HL1398] used in Western Blot (WB). GTX636838

GTX636838 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [HL1398] (GTX636838) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PI3 kinase p85 alpha antibody [HL1398] used in Western Blot (WB). GTX636838

GTX636838 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [HL1398] (GTX636838) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for PI3 kinase p85 alpha antibody [HL1398] (GTX636838). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for PI3 kinase p85 alpha antibody [HL1398] (GTX636838). Be the first to share your experience with this product.
Package List Price ($)
$ 379
$ 169
Custom Conjugations