APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PI3 kinase p85 alpha. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
phosphoinositide-3-kinase regulatory subunit 1 , AGM7 , GRB1 , IMD36 , p85 , p85-ALPHA
Background
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
Database
Research Area
DATA IMAGES
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GTX636880 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [HL1414] (GTX636880) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636880 IHC-P Image
PI3 kinase p85 alpha antibody [HL1414] detects PI3 kinase p85 alpha protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.PI3 kinase p85 alpha stained by PI3 kinase p85 alpha antibody [HL1414] (GTX636880) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636880 IHC-P Image
PI3 kinase p85 alpha antibody [HL1414] detects PI3 kinase p85 alpha protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.PI3 kinase p85 alpha stained by PI3 kinase p85 alpha antibody [HL1414] (GTX636880) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636880 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [HL1414] (GTX636880) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636880 ICC/IF Image
PI3 kinase p85 alpha antibody [HL1414] detects PI3 kinase p85 alpha protein at Golgi apparatus and cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PI3 kinase p85 alpha stained by PI3 kinase p85 alpha antibody [HL1414] (GTX636880) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.
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REFERENCE
There are currently no references for PI3 kinase p85 alpha antibody [HL1414] (GTX636880). Be the first to share your publications with this product.
REVIEW
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