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PI3 kinase p85 alpha antibody [HL1414]

Anti-PI3 kinase p85 alpha antibody [HL1414] used in Western Blot (WB). GTX636880
Anti-PI3 kinase p85 alpha antibody [HL1414] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636880
Anti-PI3 kinase p85 alpha antibody [HL1414] used in IHC (Paraffin sections) (IHC-P). GTX636880
Anti-PI3 kinase p85 alpha antibody [HL1414] used in IHC (Paraffin sections) (IHC-P). GTX636880
Anti-PI3 kinase p85 alpha antibody [HL1414] used in Western Blot (WB). GTX636880

Cat No. GTX636880

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1414

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PI3 kinase p85 alpha. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

phosphoinositide-3-kinase regulatory subunit 1 , AGM7 , GRB1 , IMD36 , p85 , p85-ALPHA

Background

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

Database

Research Area

DATA IMAGES

Anti-PI3 kinase p85 alpha antibody [HL1414] used in Western Blot (WB). GTX636880

GTX636880 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [HL1414] (GTX636880) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PI3 kinase p85 alpha antibody [HL1414] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636880

GTX636880 ICC/IF Image

PI3 kinase p85 alpha antibody [HL1414] detects PI3 kinase p85 alpha protein at Golgi apparatus and cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PI3 kinase p85 alpha stained by PI3 kinase p85 alpha antibody [HL1414] (GTX636880) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-PI3 kinase p85 alpha antibody [HL1414] used in IHC (Paraffin sections) (IHC-P). GTX636880

GTX636880 IHC-P Image

PI3 kinase p85 alpha antibody [HL1414] detects PI3 kinase p85 alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
PI3 kinase p85 alpha stained by PI3 kinase p85 alpha antibody [HL1414] (GTX636880) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PI3 kinase p85 alpha antibody [HL1414] used in IHC (Paraffin sections) (IHC-P). GTX636880

GTX636880 IHC-P Image

PI3 kinase p85 alpha antibody [HL1414] detects PI3 kinase p85 alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
PI3 kinase p85 alpha stained by PI3 kinase p85 alpha antibody [HL1414] (GTX636880) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PI3 kinase p85 alpha antibody [HL1414] used in Western Blot (WB). GTX636880

GTX636880 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [HL1414] (GTX636880) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 379
$ 169
Custom Conjugations