Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [HL1414] (GTX636880) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [HL1414] (GTX636880) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PI3 kinase p85 alpha antibody [HL1414] detects PI3 kinase p85 alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
PI3 kinase p85 alpha stained by PI3 kinase p85 alpha antibody [HL1414] (GTX636880) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PI3 kinase p85 alpha antibody [HL1414] detects PI3 kinase p85 alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
PI3 kinase p85 alpha stained by PI3 kinase p85 alpha antibody [HL1414] (GTX636880) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PI3 kinase p85 alpha antibody [HL1414] detects PI3 kinase p85 alpha protein at Golgi apparatus and cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PI3 kinase p85 alpha stained by PI3 kinase p85 alpha antibody [HL1414] (GTX636880) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.