GeneTex
United States (US)

PI3 kinase p85 alpha antibody [N2C1], Internal

GTX111068_40912_20180817_WB_shRNA_watermark_19011507_649.jpg
GTX111068_40912_20160505_WB_R_19011507_232.jpg
GTX111068_40912_20160421_WB_19011507_697.jpg
GTX111068_40912_20160120_IHC-P_M_19011507_603.jpg
GTX111068_40912_20160331_WB_M_19011507_852.jpg
GTX111068_40912_IP_19011507_820.jpg

Cat No. GTX111068

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

293T , Jurkat , K562 , THP-1 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PI3 kinase p85 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phosphoinositide-3-Kinase Regulatory Subunit 1,Agm7,Grb1,Imd36,P85,P85-Alpha,Pik3R1

Background

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX111068_40912_20180817_WB_shRNA_watermark_19011507_649.jpg

GTX111068 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111068_40912_20160505_WB_R_19011507_232.jpg

GTX111068 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111068_40912_20160421_WB_19011507_697.jpg

GTX111068 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111068_40912_20160120_IHC-P_M_19011507_603.jpg

GTX111068 IHC-P Image

PI3 kinase p85 alpha antibody [N2C1], Internal detects PIK3R1 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX111068_40912_20160331_WB_M_19011507_852.jpg

GTX111068 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111068_40912_IP_19011507_820.jpg

GTX111068 IP Image

PI3 kinase p85 alpha antibody [N2C1], Internal immunoprecipitates PI3 kinase p85 alpha protein in IP experiments.
IP samples: Jurkat whole cell extract
A. Control with 5 μg of preimmune Rabbit IgG
B. Immunoprecipitation of PI3 kinase p85 alpha protein by 5 μg PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068)
10 % SDS-PAGE
The immunoprecipitated PI3 kinase p85 alpha protein was detected by PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169