GeneTex
United States (US)

PI3 kinase p85 alpha antibody [N2C1], Internal

KO/KD
GTX111068_40912_20180817_WB_shRNA_watermark_19011507_649.jpg
GTX111068_40912_20160505_WB_R_19011507_232.jpg
GTX111068_40912_20160421_WB_19011507_697.jpg
GTX111068_40912_20160120_IHC-P_M_19011507_603.jpg
GTX111068_40912_20160331_WB_M_19011507_852.jpg
GTX111068_40912_IP_19011507_820.jpg

Cat No. GTX111068

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

293T , Jurkat , K562 , THP-1 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PI3 kinase p85 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GRB1 antibody, p85 antibody, p85-ALPHA antibody, PIK3R1 antibody, phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody, "phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) antibody", PI3K regulatory subunit alpha antibody, ptdIns-3-kinase regulatory subunit alpha antibody, phosphatidylinositol 3-kinase-associated p-85 alpha antibody, PI3-kinase subunit p85-alpha antibody, phosphatidylinositol 3-kinase regulatory subunit alpha antibody, "phosphoinositide-3-kinase, regulatory subunit 1 (alpha) antibody"

Background

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX111068_40912_20180817_WB_shRNA_watermark_19011507_649.jpg

GTX111068 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111068_40912_20160505_WB_R_19011507_232.jpg

GTX111068 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111068_40912_20160421_WB_19011507_697.jpg

GTX111068 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111068_40912_20160120_IHC-P_M_19011507_603.jpg

GTX111068 IHC-P Image

PI3 kinase p85 alpha antibody [N2C1], Internal detects PIK3R1 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX111068_40912_20160331_WB_M_19011507_852.jpg

GTX111068 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111068_40912_IP_19011507_820.jpg

GTX111068 IP Image

PI3 kinase p85 alpha antibody [N2C1], Internal immunoprecipitates PI3 kinase p85 alpha protein in IP experiments.
IP samples: Jurkat whole cell extract
A. Control with 5 μg of preimmune Rabbit IgG
B. Immunoprecipitation of PI3 kinase p85 alpha protein by 5 μg PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068)
10 % SDS-PAGE
The immunoprecipitated PI3 kinase p85 alpha protein was detected by PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf