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PI3 kinase p85 alpha antibody [N2C1], Internal

KO/KD
Anti-PI3 kinase p85 alpha antibody [N2C1], Internal used in Western Blot (WB). GTX111068
Anti-PI3 kinase p85 alpha antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX111068
Anti-PI3 kinase p85 alpha antibody [N2C1], Internal used in Western Blot (WB). GTX111068
Anti-PI3 kinase p85 alpha antibody [N2C1], Internal used in Western Blot (WB). GTX111068
Anti-PI3 kinase p85 alpha antibody [N2C1], Internal used in Western Blot (WB). GTX111068
Anti-PI3 kinase p85 alpha antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111068

Cat. No. GTX111068

Reference 10

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PI3 kinase p85 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 , 293T

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PI3 kinase p85 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177966

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoinositide-3-kinase regulatory subunit 1 , AGM7 , GRB1 , IMD36 , p85 , p85-ALPHA

Background

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PI3 kinase p85 alpha antibody [N2C1], Internal used in Western Blot (WB). GTX111068

GTX111068 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PI3 kinase p85 alpha antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX111068

GTX111068 IP Image

PI3 kinase p85 alpha antibody [N2C1], Internal immunoprecipitates PI3 kinase p85 alpha protein in IP experiments.
IP samples: Jurkat whole cell extract
A. Control with 5 μg of preimmune Rabbit IgG
B. Immunoprecipitation of PI3 kinase p85 alpha protein by 5 μg PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068)
10 % SDS-PAGE
The immunoprecipitated PI3 kinase p85 alpha protein was detected by PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-PI3 kinase p85 alpha antibody [N2C1], Internal used in Western Blot (WB). GTX111068

GTX111068 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PI3 kinase p85 alpha antibody [N2C1], Internal used in Western Blot (WB). GTX111068

GTX111068 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PI3 kinase p85 alpha antibody [N2C1], Internal used in Western Blot (WB). GTX111068

GTX111068 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PI3 kinase p85 alpha antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111068

GTX111068 IHC-P Image

PI3 kinase p85 alpha antibody [N2C1], Internal detects PIK3R1 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

YJ Shi et al. Eur J Pharmacol 2023; Sacubitril/valsartan attenuates myocardial inflammation, hypertrophy, and fibrosis in rats with heart failure with preserved ejection fraction
Jing Chen et al. Front Oncol 2022; 12 : 825484 Systemic Deficiency of PTEN Accelerates Breast Cancer Growth and Metastasis.
Yeh CC et al. Mol Med Rep 2022; 25 (2) Phytochemical?rich herbal formula ATG?125 protects against sucrose?induced gastrocnemius muscle atrophy by rescuing Akt signaling and improving mitochondrial dysfunction in young adult mice.
Application : IHC-P
Reactivity : Mouse
Lee KT et al. Aging (Albany NY) 2021; 13 (18) : 22490-22501 Nesfatin-1 facilitates IL-1β production in osteoarthritis synovial fibroblasts by suppressing miR-204-5p synthesis through the AP-1 and NF-κB pathways.
Application : WB
Reactivity : Human
Chao CC et al. Cancers (Basel) 2020; 12 (2) CXCL1/CXCR2 Paracrine Axis Contributes to Lung Metastasis in Osteosarcoma.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
YJ Shi et al. Eur J Pharmacol 2023; Sacubitril/valsartan attenuates myocardial inflammation, hypertrophy, and fibrosis in rats with heart failure with preserved ejection fraction
Jing Chen et al. Front Oncol 2022; 12:825484 Systemic Deficiency of PTEN Accelerates Breast Cancer Growth and Metastasis.
Yeh CC et al. Mol Med Rep 2022; 25 (2) Phytochemical?rich herbal formula ATG?125 protects against sucrose?induced gastrocnemius muscle atrophy by rescuing Akt signaling and improving mitochondrial dysfunction in young adult mice.
Application : IHC-P
Reactivity : Mouse
Lee KT et al. Aging (Albany NY) 2021; 13 (18):22490-22501 Nesfatin-1 facilitates IL-1β production in osteoarthritis synovial fibroblasts by suppressing miR-204-5p synthesis through the AP-1 and NF-κB pathways.
Application : WB
Reactivity : Human
Chao CC et al. Cancers (Basel) 2020; 12 (2) CXCL1/CXCR2 Paracrine Axis Contributes to Lung Metastasis in Osteosarcoma.
Application : WB
Reactivity : Human
Hu Y et al. Mol Reprod Dev 2019; MiR-8-3p regulates hyperthermia-induced lactate secretion by targeting PPP2R5B in boar Sertoli cells.
Application : WB
Reactivity : Human
Yi-Feng Yang et al. Chinese Journal of Physiology 2019; 62 (4):139-147 Adenine decreases hypertrophic effects through interleukin-18 receptor.
Application : WB
Reactivity : Rat
Huang WY et al. Curr Med Sci 2018; 38 (4):704-713 Jiao-tai-wan Up-regulates Hypothalamic and Peripheral Circadian Clock Gene Cryptochrome and Activates PI3K/AKT Signaling in Partially Sleep-deprived Rats.
Chen PH et al. PLoS One 2016; 11 (11):e0167096 the inhibition of microrna-128 on igf-1-activating mtor signaling involves in *-induced glioma cell apoptotic death.
Application : WB
Reactivity : Human
Chiu WC et al. PLoS One 2016; 11 (11):e0166453 The Synthetic β-Nitrostyrene Derivative CYT-Rx20 Inhibits Esophageal Tumor Growth and Metastasis via PI3K/AKT and STAT3 Pathways.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169