PI3 kinase p85 alpha antibody [N2C1], Internal

Cat. No. GTX111068
12
Cat. No.  GTX111068
KO/KD
Anti-PI3 kinase p85 alpha antibody [N2C1], Internal used in Western Blot (WB). GTX111068
GTX111068 WB Image
KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

1 / 6
Anti-PI3 kinase p85 alpha antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111068
GTX111068 IHC-P Image

PI3 kinase p85 alpha antibody [N2C1], Internal detects PIK3R1 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

2 / 6
Anti-PI3 kinase p85 alpha antibody [N2C1], Internal used in Western Blot (WB). GTX111068
GTX111068 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

3 / 6
Anti-PI3 kinase p85 alpha antibody [N2C1], Internal used in Western Blot (WB). GTX111068
GTX111068 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

4 / 6
Anti-PI3 kinase p85 alpha antibody [N2C1], Internal used in Western Blot (WB). GTX111068
GTX111068 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

5 / 6
Anti-PI3 kinase p85 alpha antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX111068
GTX111068 IP Image

PI3 kinase p85 alpha antibody [N2C1], Internal immunoprecipitates PI3 kinase p85 alpha protein in IP experiments.
IP samples: Jurkat whole cell extract
A. Control with 5 μg of preimmune Rabbit IgG
B. Immunoprecipitation of PI3 kinase p85 alpha protein by 5 μg PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068)
10 % SDS-PAGE
The immunoprecipitated PI3 kinase p85 alpha protein was detected by PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

6 / 6
Anti-PI3 kinase p85 alpha antibody [N2C1], Internal used in Western Blot (WB). GTX111068
Anti-PI3 kinase p85 alpha antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111068
Anti-PI3 kinase p85 alpha antibody [N2C1], Internal used in Western Blot (WB). GTX111068
Anti-PI3 kinase p85 alpha antibody [N2C1], Internal used in Western Blot (WB). GTX111068
Anti-PI3 kinase p85 alpha antibody [N2C1], Internal used in Western Blot (WB). GTX111068
Anti-PI3 kinase p85 alpha antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX111068
This PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) is a rabbit polyclonal antibody validated for detection of PI3 kinase p85 alpha by WB, IHC-P, and IP. Reactivity has been shown in Human, Mouse, and Rat. This product is cited in 12 published studies. Knockout/knockdown validation supports antibody specificity.
  • Host
    Rabbit
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Applications
    WB IHC-P IP
  • Reactivity
    Human, Mouse, Rat
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