APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat , Raji , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12
Predict Reactivity
Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human PI3 kinase p85 alpha. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885326
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
phosphoinositide-3-kinase regulatory subunit 1 , AGM7 , GRB1 , IMD36 , p85 , p85-ALPHA
Background
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX112994 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody (GTX112994) diluted at 1:500.
|
|
|
GTX112994 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody (GTX112994) diluted at 1:1000.
|
|
|
GTX112994 IHC-P Image
PI3 kinase p85 alpha antibody detects PI3 kinase p85 alpha protein at cytosol on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
PI3 kinase p85 alpha antibody (GTX112994) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
REFERENCE
REVIEW
There are currently no reviews for PI3 kinase p85 alpha antibody (GTX112994). Be the first to share your experience with this product.
