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PI3 kinase p85 alpha antibody

Comparison Orthogonal
Anti-PI3 kinase p85 alpha antibody used in Western Blot (WB). GTX132598
Anti-PI3 kinase p85 alpha antibody used in Western Blot (WB). GTX132598
Anti-PI3 kinase p85 alpha antibody used in Western Blot (WB). GTX132598

Cat. No. GTX132598

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PI3 kinase p85 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

Jurkat , Raji

Predict Reactivity

Rat, Zebrafish, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.13 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human PI3 kinase p85 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886689

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoinositide-3-kinase regulatory subunit 1 , AGM7 , GRB1 , IMD36 , p85 , p85-ALPHA

Background

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PI3 kinase p85 alpha antibody used in Western Blot (WB). GTX132598

GTX132598 WB Image

Comparison Orthogonal

Untreated (–) and treated (+) C2C12 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with PI3 kinase p85 alpha antibody (GTX132598) diluted at 1:500 and competitor's antibody (CST 4228) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-PI3 kinase p85 alpha antibody used in Western Blot (WB). GTX132598

GTX132598 WB Image

Comparison Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with PI3 kinase p85 alpha antibody (GTX132598) diluted at 1:500 and competitor's antibody (CST 4228) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-PI3 kinase p85 alpha antibody used in Western Blot (WB). GTX132598

GTX132598 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody (GTX132598) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169