GeneTex
United States (US)

PI3 kinase p85 beta antibody [GT1119]

GTX64311_20180608_IHC-P_18121411_511.jpg
GTX64311_20180608_ICCIF_1_18121411_939.jpg
GTX64311_20180608_FACS_18121411_458.jpg
GTX64311_20180608_ICCIF_18121411_652.jpg
GTX64311_20180608_WB_18121411_455.jpg

Cat No. GTX64311

Host Rabbit
Clonality Monoclonal
Clone Name GT1119
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

82 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

TBS pH7.4, 1%BSA, 40%Glycerol, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Purification

Protein A purified

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

p85BETA Antibody , p85 Antibody , P85B Antibody , MPPH Antibody , PIK3R2 Antibody , phosphoinositide-3-kinase regulatory subunit 2 Antibody

Background

Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene. [provided by RefSeq, Dec 2012]

Research Area

DATA IMAGES
GTX64311_20180608_IHC-P_18121411_511.jpg

GTX64311 IHC-P Image

IHC-P analysis of rat brain tissue using GTX64311 PI3K p85 beta antibody [GT1119].

GTX64311_20180608_ICCIF_1_18121411_939.jpg

GTX64311 ICC/IF Image

ICC/IF analysis of SHG-44 cells using GTX64311 PI3K p85 beta antibody [GT1119].
Fixation : 4% PFA
Permeabilization : 0.25% Triton X-100 in PBS
Green : Primary antibody
Blue : DAPI

GTX64311_20180608_FACS_18121411_458.jpg

GTX64311 FACS Image

FACS analysis of HeLa cells using GTX64311 PI3K p85 beta antibody [GT1119].
Blue : Primary antibody
Red : Isotype control

GTX64311_20180608_ICCIF_18121411_652.jpg

GTX64311 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX64311 PI3K p85 beta antibody [GT1119].
Fixation : 4% PFA
Permeabilization : 0.25% Triton X-100 in PBS
Green : Primary antibody
Blue : DAPI

GTX64311_20180608_WB_18121411_455.jpg

GTX64311 WB Image

WB analysis of various Samples using GTX64311 PI3K p85 beta antibody [GT1119].
Lane1 : Raji whole cell lysate
Lane2 : HeLa whole cell lysate
Lane3 : MCF-7 whole cell lysate
Lane4 : U937 whole cell lysate
Dilution : 1:1000

Package List Price ($)
$ 419