GeneTex
United States (US)

PI3 kinase p85 beta antibody [GT1119]

GTX64311_20180608_IHC-P_18121411_511.jpg
GTX64311_20180608_ICCIF_1_18121411_939.jpg
GTX64311_20180608_FACS_18121411_458.jpg
GTX64311_20180608_ICCIF_18121411_652.jpg
GTX64311_20180608_WB_18121411_455.jpg

Cat No. GTX64311

Host Rabbit
Clonality Monoclonal
Clone Name GT1119
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

82 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

TBS pH7.4, 1%BSA, 40%Glycerol, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phosphoinositide-3-Kinase Regulatory Subunit 2,Mpph,Mpph1,P85B,P85,P85-Beta,Pik3R2

Background

Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene. [provided by RefSeq, Dec 2012]

Research Area

DATA IMAGES
GTX64311_20180608_IHC-P_18121411_511.jpg

GTX64311 IHC-P Image

IHC-P analysis of rat brain tissue using GTX64311 PI3K p85 beta antibody [GT1119].

GTX64311_20180608_ICCIF_1_18121411_939.jpg

GTX64311 ICC/IF Image

ICC/IF analysis of SHG-44 cells using GTX64311 PI3K p85 beta antibody [GT1119].
Fixation : 4% PFA
Permeabilization : 0.25% Triton X-100 in PBS
Green : Primary antibody
Blue : DAPI

GTX64311_20180608_FACS_18121411_458.jpg

GTX64311 FACS Image

FACS analysis of HeLa cells using GTX64311 PI3K p85 beta antibody [GT1119].
Blue : Primary antibody
Red : Isotype control

GTX64311_20180608_ICCIF_18121411_652.jpg

GTX64311 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX64311 PI3K p85 beta antibody [GT1119].
Fixation : 4% PFA
Permeabilization : 0.25% Triton X-100 in PBS
Green : Primary antibody
Blue : DAPI

GTX64311_20180608_WB_18121411_455.jpg

GTX64311 WB Image

WB analysis of various Samples using GTX64311 PI3K p85 beta antibody [GT1119].
Lane1 : Raji whole cell lysate
Lane2 : HeLa whole cell lysate
Lane3 : MCF-7 whole cell lysate
Lane4 : U937 whole cell lysate
Dilution : 1:1000

Package List Price ($)
$ 419