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PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody

Orthogonal
Anti-PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody used in Western Blot (WB). GTX132597
Anti-PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody used in Western Blot (WB). GTX132597
Anti-PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody used in Western Blot (WB). GTX132597
Anti-PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody used in Western Blot (WB). GTX132597

Cat. No. GTX132597

Reference 19

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PI3 kinase p85 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

HepG2 (50 μM H2O2 treatment for 30 min)

Predict Reactivity

Zebrafish, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr467 of human PI3 kinase p85. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886688

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoinositide-3-kinase regulatory subunit 1 , AGM7 , GRB1 , IMD36 , p85 , p85-ALPHA

Background

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

Database

Research Area

DATA IMAGES

Anti-PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody used in Western Blot (WB). GTX132597

GTX132597 WB Image

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody (GTX132597) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody used in Western Blot (WB). GTX132597

GTX132597 WB Image

Orthogonal

Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody (GTX132597) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody used in Western Blot (WB). GTX132597

GTX132597 WB Image

Orthogonal

Untreated (–) and treated (+) C2C12 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody (GTX132597) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody used in Western Blot (WB). GTX132597

GTX132597 WB Image

Orthogonal

The data was published in the journal Int J Mol Sci in 2019. PMID: 30781615

REFERENCE

Application Reference

Nie Q et al. Sci Rep 2023; 13 (1) : 14764 PI3K/Akt signalling pathway-associated long noncoding RNA signature predicts the prognosis of laryngeal cancer patients.
P?rez-Mora S et al. Int J Mol Sci 2023; 24 (15) BFNB Enhances Hair Growth in C57BL/6 Mice through the Induction of EGF and FGF7 Factors and the PI3K-AKT-β-Catenin Pathway.
Chen M et al. iScience 2023; 26 (4) : 106524 CD36 regulates diurnal glucose metabolism and hepatic clock to maintain glucose homeostasis in mice.
Li M et al. Dis Markers 2022; 2022 : 1941077 DDTC Suppresses Ovarian Cancer Development via the PI3K/AKT/mTOR Signaling Pathway.
Application : WB
Reactivity : Human
Jing Chen et al. Front Oncol 2022; 12 : 825484 Systemic Deficiency of PTEN Accelerates Breast Cancer Growth and Metastasis.
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REVIEW

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Application Reference
Nie Q et al. Sci Rep 2023; 13 (1):14764 PI3K/Akt signalling pathway-associated long noncoding RNA signature predicts the prognosis of laryngeal cancer patients.
P?rez-Mora S et al. Int J Mol Sci 2023; 24 (15) BFNB Enhances Hair Growth in C57BL/6 Mice through the Induction of EGF and FGF7 Factors and the PI3K-AKT-β-Catenin Pathway.
Chen M et al. iScience 2023; 26 (4):106524 CD36 regulates diurnal glucose metabolism and hepatic clock to maintain glucose homeostasis in mice.
Li M et al. Dis Markers 2022; 2022:1941077 DDTC Suppresses Ovarian Cancer Development via the PI3K/AKT/mTOR Signaling Pathway.
Application : WB
Reactivity : Human
Jing Chen et al. Front Oncol 2022; 12:825484 Systemic Deficiency of PTEN Accelerates Breast Cancer Growth and Metastasis.
Guichuan Huang et al. Mol Med Rep 2021; 24 (1) Downregulation of miR?483?5p inhibits TGF?β1?induced EMT by targeting RhoGDI1 in pulmonary fibrosis.
Application : WB
Reactivity : Human
Meng Li et al. Dis Markers 2021; 2021:4304507 The Mechanism of Xiaoyao San in the Treatment of Ovarian Cancer by Network Pharmacology and the Effect of Stigmasterol on the PI3K/Akt Pathway.
Application : WB
Reactivity : Human
Yang L et al. Front Pharmacol 2021; 12:763181 Effect of Neurorepair for Motor Functional Recovery Enhanced by Total Saponins From Trillium tschonoskii Maxim. Treatment in a Rat Model of Focal Ischemia.
Chiou TT et al. Biomed Pharmacother 2021; 144:112349 Rapamycin attenuates PLA2R activation-mediated podocyte apoptosis via the PI3K/AKT/mTOR pathway.
Application : WB
Luo X et al. Cell Death Dis 2021; 12 (4):328 The fatty acid receptor CD36 promotes HCC progression through activating Src/PI3K/AKT axis-dependent aerobic glycolysis.
Application : WB
Reactivity : Mouse
Li J et al. J Ethnopharmacol 2020; 257:112796 Effective-component compatibility of Bufei Yishen formula II inhibits mucus hypersecretion of chronic obstructive pulmonary disease rats by regulating EGFR/PI3K/mTOR signaling.
Application : WB
Reactivity : Rat
Chao CC et al. Cancers (Basel) 2020; 12 (2) CXCL1/CXCR2 Paracrine Axis Contributes to Lung Metastasis in Osteosarcoma.
Application : WB
Reactivity : Human
Li H et al. Neurotox Res 2020; 37 (4):996-1008 Aluminum-Induced Synaptic Plasticity Impairment via PI3K-Akt-mTOR Signaling Pathway.
Application : WB
Reactivity : Rat
Jau?Tien Lin et al. Journal of Food Biochemistry 2019; (Epub) Litchi (Litchi chinensis Sonn.) flower proanthocyanidin fraction exhibited protective efficacy to suppress nickel?induced expression for vascular endothelial growth factor in HepG2 cells.
Application : WB
Reactivity : Human
Fang L et al. Int J Mol Sci 2019; 20 (4) IgE Downregulates PTEN through MicroRNA-21-5p and Stimulates Airway Smooth Muscle Cell Remodeling.
Application : ICC/IF
Reactivity : Human
Huifang Yan et al. Evidence-Based Complementary and Alternative Medicine 2018; 2018:Article ID 1508637 Inhibition of Aortic Intimal Hyperplasia and Vascular Smooth Muscle Proliferation and Extracellular Matrix Protein Expressions by Astragalus–Angelica Combination.
Application : WB
Reactivity : Rat
Lin JT et al. Journal of Functional Foods 2018; 47:343-349 Protective effect and mechanism of Muntingia calabura Linn. fruit ethanolic extract against vascular endothelial growth factor production in nickel-stimulated hepatocellular carcinoma cells.
Application : WB
Reactivity : Human
Chiu WC et al. PLoS One 2016; 11 (11):e0166453 The Synthetic β-Nitrostyrene Derivative CYT-Rx20 Inhibits Esophageal Tumor Growth and Metastasis via PI3K/AKT and STAT3 Pathways.
Application : WB
Reactivity : Human
Ali NA et al. Breast Cancer Res 2014; 16 (5):437 Profiling the tyrosine phosphoproteome of different mouse mammary tumour models reveals distinct, model-specific signalling networks and conserved oncogenic pathways.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169