GeneTex
United States (US)

PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody

Orthogonal
GTX132597_42431_20161124_WB_CIP_18121410_435.jpg

Cat No. GTX132597

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Zebrafish, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 50% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AGM7 antibody antibody , GRB1 antibody antibody , IMD36 antibody antibody , PIK3R1 antibody antibody , p85 antibody antibody , p85-ALPHA antibody antibody , phosphoinositide-3-kinase regulatory subunit 1 antibody antibody

Background

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

Database

Research Area

DATA IMAGES
GTX132597_42431_20161124_WB_CIP_18121410_435.jpg

GTX132597 WB Image

Orthogonal

Untreated (–) and treated (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody (GTX132597) diluted at 1:500.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf