GeneTex
United States (US)

PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody

PI3K-P85-Phospho-Tyr467-Antibody-GTX50858-WB-1_18121411_729.jpg

Cat No. GTX50858

Host Rabbit
Clonality Polyclonal
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Calculated MW

85 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 467 (L-Y(p)-E-E-Y) derived from Human PI3K P85.

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GRB1, p85, p85-ALPHA, PIK3R1, phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha), PI3K regulatory subunit alpha, ptdIns-3-kinase regulatory subunit alpha, phosphatidylinositol 3-kinase-associated p-85 alpha, PI3-kinase subunit p85-alpha, phosphatidylinositol 3-kinase regulatory subunit alpha, phosphoinositide-3-kinase, regulatory subunit 1 (alpha), DKFZp686P05226, FLJ41892, p55, p55-GAMMA, PIK3R3, ptdIns-3-kinase regulatory subunit p55-gamma, ptdIns-3-kinase regulatory subunit gamma, p55PIK, phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 3 (p55, gamma), phosphoinositide-3-kinase, regulatory subunit, polypeptide 3 (p55, gamma), phosphatidylinositol 3-kinase regulatory subunit gamma, PI3-kinase subunit p55-gamma, PI3K regulatory subunit gamma, 100% homology to SWISS-PROT Q92569, phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma, PI3-kinase regulatory subunit gamma, phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma), phosphoinositide-3-kinase, regulatory subunit 3 (gamma), P85B, p85-BETA, PIK3R2, phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta, phosphoinositide-3-kinase, regulatory subunit, polypeptide 2 (p85 beta), PI3K regulatory subunit beta, phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta), PI3-kinase subunit p85-beta, phosphoinositide-3-kinase, regulatory subunit 2 (p85 beta), phosphatidylinositol 3-kinase regulatory subunit beta, ptdIns-3-kinase regulatory subunit p85-beta, PI3-kinase regulatory subunit beta, ptdIns-3-kinase regulatory subunit beta, phosphoinositide-3-kinase, regulatory subunit 2 (beta)

Background

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1./Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.

Database

Research Area

DATA IMAGES
PI3K-P85-Phospho-Tyr467-Antibody-GTX50858-WB-1_18121411_729.jpg

GTX50858 WB Image

Western blot analysis of extracts from 293 cells untreated or treated with sorbitol using PI3K P85 (phospho-Tyr467) Antibody (GTX50858).

REFERENCE