GeneTex
United States (US)

PI4 kinase alpha antibody [C1C2], Internal

GTX107441_40408_IHC_19011506_243.jpg
GTX107441_42795_20170330_WB_19011506_211.jpg
GTX107441_42795_20170906_IFA_19011506_430.jpg

Cat No. GTX107441

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

231 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PI4 kinase alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phosphatidylinositol 4-Kinase Alpha,Pi4K-Alpha,Pik4Ca,Pmgycha,Pi4K230,Pi4Ka

Background

This gene encodes a phosphatidylinositol (PI) 4-kinase which catalyzes the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. The mammalian PI 4-kinases have been classified into two types, II and III, based on their molecular mass, and modulation by detergent and adenosine. The protein encoded by this gene is a type III enzyme that is not inhibited by adenosine. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

DATA IMAGES
GTX107441_40408_IHC_19011506_243.jpg

GTX107441 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using PI4 kinase alpha(GTX107441) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107441_42795_20170330_WB_19011506_211.jpg

GTX107441 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI4 kinase alpha antibody [C1C2], Internal (GTX107441) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107441_42795_20170906_IFA_19011506_430.jpg

GTX107441 ICC/IF Image

PI4 kinase alpha antibody [C1C2], Internal detects PI4 kinase alpha protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PI4 kinase alpha protein stained by PI4 kinase alpha antibody [C1C2], Internal (GTX107441) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169