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PIAS1 antibody [GT1323]

Recombinant antibody
Anti-PIAS1 antibody [GT1323] used in Western Blot (WB). GTX03235
Anti-PIAS1 antibody [GT1323] used in Western Blot (WB). GTX03235
Anti-PIAS1 antibody [GT1323] used in Western Blot (WB). GTX03235

Cat. No. GTX03235

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1323

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($439)
See all PIAS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human PIAS1.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein inhibitor of activated STAT 1 , DDXBP1 , GBP , GU/RH-II , ZMIZ3

Cellular Localization

Nucleus speckle

Background

This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. This protein plays a central role as a transcriptional coregulator of numerous cellular pathways includign the STAT1 and nuclear factor kappaB pathways. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Database

Research Area

DATA IMAGES

Anti-PIAS1 antibody [GT1323] used in Western Blot (WB). GTX03235

GTX03235 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PIAS1 antibody [GT1323] (GTX03235) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PIAS1 antibody [GT1323] used in Western Blot (WB). GTX03235

GTX03235 WB Image

WB analysis of mouse lung tissue lysate using GTX03235 PIAS1 antibody [GT1323].
Dilution : 1:1000
Loading : 25μg per lane

Anti-PIAS1 antibody [GT1323] used in Western Blot (WB). GTX03235

GTX03235 WB Image

WB analysis of various samples using GTX03235 PIAS1 antibody [GT1323].
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 439