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PIAS1 antibody

Anti-PIAS1 antibody used in Western Blot (WB). GTX31410
Anti-PIAS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31410
Anti-PIAS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31410

Cat. No. GTX31410

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all PIAS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

Human Kidney Tissue Lysate(GTX29995)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PIAS1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human PIAS1.The immunogen is located within the last 50 amino acids of PIAS1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein inhibitor of activated STAT 1 , DDXBP1 , GBP , GU/RH-II , ZMIZ3

Cellular Localization

Nucleus speckle

Background

This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the DNA binding activity, binds to Gu protein/RNA helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PIAS1 antibody used in Western Blot (WB). GTX31410

GTX31410 WB Image

WB analysis of human kidney tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX31410 PIAS1 antibody.
Working concentration : 1 μg/ml

Anti-PIAS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31410

GTX31410 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX31410 PIAS1 antibody.
Working concentration : 20 μg/ml

Anti-PIAS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31410

GTX31410 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX31410 PIAS1 antibody.
Working concentration : 5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399