GeneTex
United States (US)

PIAS2 (aa185-196) antibody, Internal

GTX88188_2500_WB_18121411_422.jpg

Cat No. GTX88188

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88188-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both reported isoforms (NP_775298.1; NP_004662.2).

Predict Reactivity

Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence PQQVREICISRD, from the internal region of the protein sequence according to NP_775298.1; NP_004662.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Inhibitor Of Activated Stat 2,Arip3,Dip,Miz1,Piasx,Siz2,Zmiz4,Pias2

Cellular Localization

Colocalizes at least partially with promyelocytic leukemia nuclear bodies.

Background

This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Isoforms of the encoded protein enhance the sumoylation of specific target proteins including the p53 tumor suppressor protein, c-Jun, and the androgen receptor. A pseudogene of this gene is located on the short arm of chromosome 4. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. [provided by RefSeq, Aug 2011]

Database

DATA IMAGES
GTX88188_2500_WB_18121411_422.jpg

GTX88188 WB Image

WB analysis of human peripheral lymphocytes lysate using GTX88188 PIAS2 (aa185-196) antibody, Internal.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329