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PIAS2 antibody

Anti-PIAS2 antibody used in Western Blot (WB). GTX115180
Anti-PIAS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX115180
Anti-PIAS2 antibody used in Western Blot (WB). GTX115180

Cat No. GTX115180

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , THP-1 , HL-60 , NCI-H929 , NIH-3T3 , JC , BCL-1

Predict Reactivity

Rat, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PIAS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162657

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein inhibitor of activated STAT 2 , ARIP3 , DIP , MIZ1 , PIASX , SIZ2 , ZMIZ4

Cellular Localization

Nucleus speckle

Background

This gene encodes a protein involved in the regulation of transcription factors involved in MAP kinase signaling. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. Two alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PIAS2 antibody used in Western Blot (WB). GTX115180

GTX115180 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX115180 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PIAS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX115180

GTX115180 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using PIAS2(GTX115180) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PIAS2 antibody used in Western Blot (WB). GTX115180

GTX115180 WB Image

Sample (30 μg of whole cell lysate)
A: Jurkat
B: Raji
C: K562
D: THP-1
E: HL-60
F: NCI-H929
7.5% SDS PAGE
GTX115180 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169