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PIAS2 antibody

Anti-PIAS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31411
Anti-PIAS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31411
Anti-PIAS2 antibody used in Western Blot (WB). GTX31411

Cat. No. GTX31411

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all PIAS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

Rat Brain Tissue Lysate(GTX27942)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PIAS2 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human PIAS2.The immunogen is located within amino acids 110 - 160 of PIAS2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein inhibitor of activated STAT 2 , ARIP3 , DIP , MIZ1 , PIASX , SIZ2 , ZMIZ4

Cellular Localization

Nucleus speckle,Nucleus

Background

This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Isoforms of the encoded protein enhance the sumoylation of specific target proteins including the p53 tumor suppressor protein, c-Jun, and the androgen receptor. A pseudogene of this gene is located on the short arm of chromosome 4. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-PIAS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31411

GTX31411 IHC-P Image

IHC-P analysis of rat brain tissue using GTX31411 PIAS2 antibody.
Working concentration : 20 μg/ml

Anti-PIAS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31411

GTX31411 IHC-P Image

IHC-P analysis of rat brain tissue using GTX31411 PIAS2 antibody.
Working concentration : 5 μg/ml

Anti-PIAS2 antibody used in Western Blot (WB). GTX31411

GTX31411 WB Image

WB analysis of rat brain tissue lysate using GTX31411 PIAS2 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399