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PIAS3 antibody, C-term

Anti-PIAS3 antibody, C-term used in Western Blot (WB). GTX77910
Anti-PIAS3 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX77910

Cat. No. GTX77910

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all PIAS3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a C-terminal region of Human PIAS3

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_424858

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein inhibitor of activated STAT 3 , ZMIZ5

Cellular Localization

Cytoplasm,Nucleus,Nucleus speckle

Background

This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PIAS3 antibody, C-term used in Western Blot (WB). GTX77910

GTX77910 WB Image

WB analysis of HepG2 cells using GTX77910 PIAS3 antibody at 1.0-2.0μg/ml.

Anti-PIAS3 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX77910

GTX77910 IHC-P Image

IHC-P analysis of human liver tissue using GTX77910 PIAS3 antibody at 4.0-8.0μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399