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PIAS3 antibody

Anti-PIAS3 antibody used in IHC (Paraffin sections) (IHC-P). GTX31412
Anti-PIAS3 antibody used in Western Blot (WB). GTX31412

Cat No. GTX31412

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

K562 Cell Lysate(GTX27911)

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PIAS3 antibody was raised against a 12 amino acid synthetic peptide near the carboxy terminus of human PIAS3.The immunogen is located within amino acids 410 - 460 of PIAS3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein inhibitor of activated STAT 3 , ZMIZ5

Cellular Localization

Cytoplasm,Nucleus,Nucleus speckle

Background

This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PIAS3 antibody used in IHC (Paraffin sections) (IHC-P). GTX31412

GTX31412 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX31412 PIAS3 antibody.
Working concentration : 5 μg/ml

Anti-PIAS3 antibody used in Western Blot (WB). GTX31412

GTX31412 WB Image

WB analysis of K562 cell lysate using GTX31412 PIAS3 antibody.
Working concentration : 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339