PIASy antibody detects PIASy protein at nucleus in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
PIASy antibody (GTX110497) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PIASy antibody detects PIASy protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human PIAS4-transfected 293T cells
10% SDS-PAGE
PIASy antibody (GTX110497) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PIASy(GTX110497) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Immunohistochemical analysis of paraffin-embedded DLD1 Xenograft, using PIAS4(GTX110497) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min