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PIASy antibody

Anti-PIASy antibody used in IHC (Paraffin sections) (IHC-P). GTX110497
Anti-PIASy antibody used in IHC (Paraffin sections) (IHC-P). GTX110497
Anti-PIASy antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110497
Anti-PIASy antibody used in Western Blot (WB). GTX110497

Cat. No. GTX110497

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PIASy products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

human PIAS4-transfected 293T cells

Predict Reactivity

Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PIASy. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951227

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein inhibitor of activated STAT 4 , PIAS-gamma , PIASY , Piasg , ZMIZ6

Cellular Localization

Nucleus

Background

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Promotes PARK7 sumoylation. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations.

Database

Research Area

DATA IMAGES

Anti-PIASy antibody used in IHC (Paraffin sections) (IHC-P). GTX110497

GTX110497 IHC-P Image

PIASy antibody detects PIASy protein at nucleus in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
PIASy antibody (GTX110497) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PIASy antibody used in IHC (Paraffin sections) (IHC-P). GTX110497

GTX110497 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD1 Xenograft, using PIAS4(GTX110497) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PIASy antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110497

GTX110497 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PIASy(GTX110497) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-PIASy antibody used in Western Blot (WB). GTX110497

GTX110497 WB Image

PIASy antibody detects PIASy protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human PIAS4-transfected 293T cells
10% SDS-PAGE
PIASy antibody (GTX110497) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169