PIASy antibody detects PIAS4 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PIAS4 protein stained by PIASy antibody (GTX116181) diluted at 1:500.
Blue: Hoechst 33342 staining.

PIASy antibody detects PIASy protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human PIAS4-transfected 293T cells
12 % SDS-PAGE
PIASy antibody (GTX116181) dilution: 1:1000

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using PIASy(GTX116181) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min