GeneTex

  • Country / Location Selection

United States (US)

PIASy antibody

Anti-PIASy antibody used in Western Blot (WB). GTX13795
Anti-PIASy antibody used in Western Blot (WB). GTX13795

Cat. No. GTX13795

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all PIASy products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 3 - 5 μg/ml
ICC/IF 1:10 - 1:2000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This polyclonal antibody was developed against a mixture of synthetic peptides corresponding to amino acids 46-60 (KKIKELYETRYAKKNC) and 187-198 (YSDTSCPQEDQY) of human PIASg; GenBank no. AAD45155.1|AF164437_1. These sequences are 95% and 100% homologous in mouse and rat.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein inhibitor of activated STAT 4 , PIAS-gamma , PIASY , Piasg , ZMIZ6

Background

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Promotes PARK7 sumoylation. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations.

Database

Research Area

DATA IMAGES

Anti-PIASy antibody used in Western Blot (WB). GTX13795

GTX13795 WB Image

WB analysis of Jurkat cell lysate using GTX13795 PIASy antibody.
Dilution : 2 μg/ml

Anti-PIASy antibody used in Western Blot (WB). GTX13795

GTX13795 WB Image

WB analysis of Jurkat cell lysate using GTX13795 PIASy antibody.
Dilution : 5 μg/ml

REFERENCE

There are currently no references for PIASy antibody (GTX13795). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for PIASy antibody (GTX13795). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399