GeneTex
United States (US)

PICK1 antibody

Cat No. GTX15001

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Mouse, Rat
APPLICATION

Application Note

WB: Use at a dilution of 1/500 - 1/1000. Predicted molecular weight: 50 kDa. Optimal dilutions/concentrations should be determined by the end user.

Positive Control

RIPA lysates from rat brain microsomal preparation

Specificity/Sensitivity

Recognizes PICK1.
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.05% Sodium Azide; Constituents: 5mg/ml BSA, 0.15M Sodium Chloride, 50mM Glycine, 0.2mM Tris, 0.1mM EDTA. pH 7.4

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Mouse

Immunogen

6xhis tagged fusion protein: EKMELLDQKH VQDIVFQLQR FVSTMSKYYN DCYAVLQDAD VFPIEVDLAH TTLAYGPNQG SFTDGEEEDE EEEDGAAREV SKDACGATGP TDKGGSWCDS, corresponding to C terminal amino acids 317-416 of Mouse PICK1.

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Interacting With C Kinase 1,Prkcabp,Pick1

Cellular Localization

Cytoplasmic; concentrated in the perinuclear region. Also present at excitatory synapses.

Background

PSD-95/DLG/ZO-1 (PDZ) domain-containing proteins play a central role in synaptic membrane protein localization. The protein interacting with protein kinase C (PICK 1) is a synaptic PDZ domain protein that also contains a coiled-coil and acidic domain. Studies indicate that PICK 1 functions as a targeting and transport protein and has been shown to interact with protein kinase C alpha (PKC alpha), AMPA-type glutamate receptors, and several other membrane receptors via its PDZ domain. The interaction of PICK 1 with PKC alpha is highly dependent on the activation of the kinase. It appears that PICK 1 directs the activated form of PKC alpha to membrane anchored GluR2. Once phosphorylated by PKC alpha, GluR2 is released from the synaptic anchor proteins. Once released, GluR2 is transported from the synaptic membrane in a PICK 1-dependent manner.

Research Area

Package List Price ($)
$ 289