GeneTex
United States (US)

PIGA antibody

GTX102597_40128_WB_M_19011506_321.jpg

Cat No. GTX102597

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

NIH-3T3

Predict Reactivity

Human, Rat, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1%BSA, 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PIGA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GPI3 antibody, PIG-A antibody, PIGA antibody, phosphatidylinositol N-acetylglucosaminyltransferase subunit A antibody, GPI anchor biosynthesis antibody, GLCNAC-PI synthesis protein antibody, class A GlcNAc-inositol phospholipid assembly protein antibody, "phosphatidylinositol-glycan biosynthesis, class A protein antibody", "phosphatidylinositol glycan anchor biosynthesis, class A antibody"

Cellular Localization

Endoplasmic reticulum membrane; Single-pass membrane protein

Background

This gene encodes a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene. Alternate splice variants have been characterized. [provided by RefSeq]
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Database

DATA IMAGES
GTX102597_40128_WB_M_19011506_321.jpg

GTX102597 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX102597 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169