GeneTex
United States (US)

PIGK antibody [N1C2]

GTX105967_39953_IHC_19011506_862.jpg
GTX105967_39953_WB_M_brain_19011506_434.jpg
GTX105967_WB_19011506_252.jpg

Cat No. GTX105967

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

293T , mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PIGK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GPI8 antibody, MGC22559 antibody, PIGK antibody, GPI8 homolog antibody, phosphatidylinositol-glycan biosynthesis class K protein antibody, PIG-K antibody, GPI transamidase subunit antibody, "phosphatidylinositol glycan, class K antibody", GPI-anchor transamidase antibody, "phosphatidylinositol glycan anchor biosynthesis, class K antibody"

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type I membrane protein

Background

This gene encodes a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. [provided by RefSeq]

Database

DATA IMAGES
GTX105967_39953_IHC_19011506_862.jpg

GTX105967 IHC-P Image

PIGK antibody [N1C2] detects PIGK protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
PIGK antibody [N1C2] (GTX105967) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105967_39953_WB_M_brain_19011506_434.jpg

GTX105967 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX105967 diluted at 1:1000

GTX105967_WB_19011506_252.jpg

GTX105967 WB Image

Sample(30 ug whole cell lysate)
A:293T
10% SDS PAGE
GTX105967 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169