GeneTex
  • Country / Location Selection

United States (US)

PIKfyve antibody

Anti-PIKfyve antibody used in Western Blot (WB). GTX34124

Cat No. GTX34124

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

237 kDa. ( Note )

Specificity/Sensitivity

PIP5KIII Polyclonal Antibody detects endogenous levels of PIP5KIII protein.

PROPERTIES

Form

Liquid

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthesized peptide derived from the N-terminal region of human PIP5KIII at AA range: 40-120.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Phosphoinositide Kinase, Fyve-Type Zinc Finger Containing , Cfd , Fab1 , Hel37 , Pip5K , Pip5K3 , Zfyve29 , Pikfyve

Background

Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. Humans have multiple PtdIns proteins that differ by the degree and position of phosphorylation of the inositol ring. This gene encodes an enzyme (PIKfyve; also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) that phosphorylates the D-5 position in PtdIns and phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns(3,5)biphosphate. The D-5 position also can be phosphorylated by type I PtdIns4P-5-kinases (PIP5Ks) that are encoded by distinct genes and preferentially phosphorylate D-4 phosphorylated PtdIns. In contrast, PIKfyve preferentially phosphorylates D-3 phosphorylated PtdIns. In addition to being a lipid kinase, PIKfyve also has protein kinase activity. PIKfyve regulates endomembrane homeostasis and plays a role in the biogenesis of endosome

Database

Research Area

DATA IMAGES

Anti-PIKfyve antibody used in Western Blot (WB). GTX34124

GTX34124 WB Image

Western Blot (WB) analysis of specific cells using PIP5KIII Polyclonal Antibody.

REFERENCE

There are currently no references for PIKfyve antibody (GTX34124). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for PIKfyve antibody (GTX34124). Be the first to share your experience with this product.