APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 110-150 of human PIM1.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
PIM,PIM1,Pim1 protooncogene, serine/threonine kinase,Pim-1 proto-oncogene, serine/threonine kinase
Cellular Localization
Cytoplasm,Nucleus,Cell membrane
Background
The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).[provided by RefSeq, Aug 2011]
Database
Research Area
DATA IMAGES
![Anti-PIM1 antibody [GT1192] used in Western Blot (WB). GTX00954](/templates/default/js/lightbox/images/loading.gif "Anti-PIM1 antibody [GT1192] used in Western Blot (WB). GTX00954")
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GTX00954 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PIM1 antibody [GT1192] (GTX00954) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 24040307
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GTX00954 IHC-P Image
IHC-P analysis of mouse brain tissue section using GTX00954 PIM1 antibody [GT1192].
Dilution : 1:100
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GTX00954 WB Image
WB analysis of 22Rv1 whole cell lysate using GTX00954 PIM1 antibody [GT1192].
Dilution : 1:1000
Loading : 25 μg
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GTX00954 IHC-P Image
IHC-P analysis of rat ovary tissue section using GTX00954 PIM1 antibody [GT1192].
Dilution : 1:100
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GTX00954 IHC-P Image
IHC-P analysis of human colon tissue section using GTX00954 PIM1 antibody [GT1192].
Dilution : 1:100
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REFERENCE
There are currently no references for PIM1 antibody [GT1192] (GTX00954). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PIM1 antibody [GT1192] (GTX00954). Be the first to share your experience with this product.
![Anti-PIM1 antibody [GT1192] used in Western Blot (WB). GTX00954](/upload/website/prouct_img/small/GTX00954/GTX00954_4000000175_20200410_WB_w_23053121_688.webp "Anti-PIM1 antibody [GT1192] used in Western Blot (WB). GTX00954")
![Anti-PIM1 antibody [GT1192] used in IHC (Paraffin sections) (IHC-P). GTX00954](/upload/website/prouct_img/small/GTX00954/GTX00954_20200508_IHC-P_w_23053121_496.webp "Anti-PIM1 antibody [GT1192] used in IHC (Paraffin sections) (IHC-P). GTX00954")
![Anti-PIM1 antibody [GT1192] used in Western Blot (WB). GTX00954](/upload/website/prouct_img/small/GTX00954/GTX00954_20200508_WB_w_23053121_371.webp "Anti-PIM1 antibody [GT1192] used in Western Blot (WB). GTX00954")
![Anti-PIM1 antibody [GT1192] used in IHC (Paraffin sections) (IHC-P). GTX00954](/upload/website/prouct_img/small/GTX00954/GTX00954_20200508_IHC-P_2_w_23053121_118.webp "Anti-PIM1 antibody [GT1192] used in IHC (Paraffin sections) (IHC-P). GTX00954")
![Anti-PIM1 antibody [GT1192] used in IHC (Paraffin sections) (IHC-P). GTX00954](/upload/website/prouct_img/small/GTX00954/GTX00954_20200508_IHC-P_1_w_23053121_102.webp "Anti-PIM1 antibody [GT1192] used in IHC (Paraffin sections) (IHC-P). GTX00954")
![Anti-PIM1 antibody [GT1192] used in Western Blot (WB). GTX00954](/upload/website/prouct_img/normal/GTX00954/GTX00954_4000000175_20200410_WB_w_23053121_688.webp "Anti-PIM1 antibody [GT1192] used in Western Blot (WB). GTX00954")
![Anti-PIM1 antibody [GT1192] used in IHC (Paraffin sections) (IHC-P). GTX00954](/upload/website/prouct_img/normal/GTX00954/GTX00954_20200508_IHC-P_w_23053121_496.webp "Anti-PIM1 antibody [GT1192] used in IHC (Paraffin sections) (IHC-P). GTX00954")
![Anti-PIM1 antibody [GT1192] used in Western Blot (WB). GTX00954](/upload/website/prouct_img/normal/GTX00954/GTX00954_20200508_WB_w_23053121_371.webp "Anti-PIM1 antibody [GT1192] used in Western Blot (WB). GTX00954")
![Anti-PIM1 antibody [GT1192] used in IHC (Paraffin sections) (IHC-P). GTX00954](/upload/website/prouct_img/normal/GTX00954/GTX00954_20200508_IHC-P_2_w_23053121_118.webp "Anti-PIM1 antibody [GT1192] used in IHC (Paraffin sections) (IHC-P). GTX00954")
![Anti-PIM1 antibody [GT1192] used in IHC (Paraffin sections) (IHC-P). GTX00954](/upload/website/prouct_img/normal/GTX00954/GTX00954_20200508_IHC-P_1_w_23053121_102.webp "Anti-PIM1 antibody [GT1192] used in IHC (Paraffin sections) (IHC-P). GTX00954")