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PIM2 antibody [9A5]

Anti-PIM2 antibody [9A5] used in Flow cytometry (FACS). GTX83892
Anti-PIM2 antibody [9A5] used in Western Blot (WB). GTX83892
Anti-PIM2 antibody [9A5] used in Immunoprecipitation (IP). GTX83892
Anti-PIM2 antibody [9A5] used in Flow cytometry (FACS). GTX83892
Anti-PIM2 antibody [9A5] used in Flow cytometry (FACS). GTX83892
Anti-PIM2 antibody [9A5] used in IHC (Paraffin sections) (IHC-P). GTX83892
Anti-PIM2 antibody [9A5] used in IHC (Paraffin sections) (IHC-P). GTX83892
Anti-PIM2 antibody [9A5] used in IHC (Paraffin sections) (IHC-P). GTX83892
Anti-PIM2 antibody [9A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83892

Cat No. GTX83892

Host

Mouse

Clonality

Monoclonal

Clone Name

9A5

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P, FACS, IP

Reactivity

Human
Package
100 μl ($389)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
IP 2ug/500ul
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human PIM2 (NP_006866) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_10726848

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Pim-2 proto-oncogene, serine/threonine kinase

Background

Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappaB kinase/NF-kappaB cascade | this process requires phosphorylation of MAP3K8/COT. Prevents apoptosis induced by growth factor withdrawal via inhibition of caspase-3 activation, and via phosphorylation of pro-apoptotic proteins. Inhibits BAD-induced cell death via phosphorylation of BAD. PIM2-mediated cell survival is glucose-dependent but independent of several AKT regulators such as PI3K, HSP-90 and TOR, indicating that PIM2 and PI3K/AKT/TOR function via distinct pathways. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.

Database

Research Area

DATA IMAGES

Anti-PIM2 antibody [9A5] used in Flow cytometry (FACS). GTX83892

GTX83892 FACS Image

FACS analysis of HEK293T cells transfected with either PIM2 plasmid(Red) or empty vector control plasmid(Blue) using GTX83892 PIM2 antibody [9A5].

Anti-PIM2 antibody [9A5] used in Western Blot (WB). GTX83892

GTX83892 WB Image

WB analysis of HEK293T cells transfected with PIM2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83892 PIM2 antibody [9A5].
Loading : 5 ug per lane

Anti-PIM2 antibody [9A5] used in Immunoprecipitation (IP). GTX83892

GTX83892 IP Image

IP analysis of DDDDK tagged PIM2 overexpressed HEK293T lysate using GTX83892 PIM2 antibody [9A5].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

Anti-PIM2 antibody [9A5] used in Flow cytometry (FACS). GTX83892

GTX83892 FACS Image

FACS analysis of HeLa cells using GTX83892 PIM2 antibody [9A5].
Red : Primary antibody
Blue : Negative control antibody

Anti-PIM2 antibody [9A5] used in Flow cytometry (FACS). GTX83892

GTX83892 FACS Image

FACS analysis of Jurkat cells using GTX83892 PIM2 antibody [9A5].
Red : Primary antibody
Blue : Negative control antibody

Anti-PIM2 antibody [9A5] used in IHC (Paraffin sections) (IHC-P). GTX83892

GTX83892 IHC-P Image

IHC-P analysis of human liver tissue using GTX83892 PIM2 antibody [9A5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-PIM2 antibody [9A5] used in IHC (Paraffin sections) (IHC-P). GTX83892

GTX83892 IHC-P Image

IHC-P analysis of human colon tissue using GTX83892 PIM2 antibody [9A5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-PIM2 antibody [9A5] used in IHC (Paraffin sections) (IHC-P). GTX83892

GTX83892 IHC-P Image

IHC-P analysis of human prostate tissue using GTX83892 PIM2 antibody [9A5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-PIM2 antibody [9A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83892

GTX83892 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with PIM2 plasmid using GTX83892 PIM2 antibody [9A5].

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 389