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PIM2 antibody [9A5]

Overexpression
Anti-PIM2 antibody [9A5] used in IHC (Paraffin sections) (IHC-P). GTX83892
Anti-PIM2 antibody [9A5] used in Flow cytometry (FACS). GTX83892
Anti-PIM2 antibody [9A5] used in Immunoprecipitation (IP). GTX83892
Anti-PIM2 antibody [9A5] used in Flow cytometry (FACS). GTX83892
Anti-PIM2 antibody [9A5] used in IHC (Paraffin sections) (IHC-P). GTX83892
Anti-PIM2 antibody [9A5] used in IHC (Paraffin sections) (IHC-P). GTX83892
Anti-PIM2 antibody [9A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83892
Anti-PIM2 antibody [9A5] used in Western Blot (WB). GTX83892
Anti-PIM2 antibody [9A5] used in Flow cytometry (FACS). GTX83892

Cat. No. GTX83892

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 9A5

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS, IP

Reactivity

 Human
Package
100 μl ($519)
See all PIM2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
IP 2ug/500ul
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human PIM2 (NP_006866) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_10726848

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Pim-2 proto-oncogene, serine/threonine kinase

Background

Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappaB kinase/NF-kappaB cascade | this process requires phosphorylation of MAP3K8/COT. Prevents apoptosis induced by growth factor withdrawal via inhibition of caspase-3 activation, and via phosphorylation of pro-apoptotic proteins. Inhibits BAD-induced cell death via phosphorylation of BAD. PIM2-mediated cell survival is glucose-dependent but independent of several AKT regulators such as PI3K, HSP-90 and TOR, indicating that PIM2 and PI3K/AKT/TOR function via distinct pathways. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.

Database

Research Area

DATA IMAGES

Anti-PIM2 antibody [9A5] used in IHC (Paraffin sections) (IHC-P). GTX83892

GTX83892 IHC-P Image

IHC-P analysis of human colon tissue using GTX83892 PIM2 antibody [9A5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-PIM2 antibody [9A5] used in Flow cytometry (FACS). GTX83892

GTX83892 FACS Image

FACS analysis of HEK293T cells transfected with either PIM2 plasmid(Red) or empty vector control plasmid(Blue) using GTX83892 PIM2 antibody [9A5].

Anti-PIM2 antibody [9A5] used in Immunoprecipitation (IP). GTX83892

GTX83892 IP Image

IP analysis of DDDDK tagged PIM2 overexpressed HEK293T lysate using GTX83892 PIM2 antibody [9A5].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

Anti-PIM2 antibody [9A5] used in Flow cytometry (FACS). GTX83892

GTX83892 FACS Image

FACS analysis of Jurkat cells using GTX83892 PIM2 antibody [9A5].
Red : Primary antibody
Blue : Negative control antibody

Anti-PIM2 antibody [9A5] used in IHC (Paraffin sections) (IHC-P). GTX83892

GTX83892 IHC-P Image

IHC-P analysis of human liver tissue using GTX83892 PIM2 antibody [9A5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-PIM2 antibody [9A5] used in IHC (Paraffin sections) (IHC-P). GTX83892

GTX83892 IHC-P Image

IHC-P analysis of human prostate tissue using GTX83892 PIM2 antibody [9A5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-PIM2 antibody [9A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83892

GTX83892 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with PIM2 plasmid using GTX83892 PIM2 antibody [9A5].

Anti-PIM2 antibody [9A5] used in Western Blot (WB). GTX83892

GTX83892 WB Image

Overexpression

WB analysis of HEK293T cells transfected with PIM2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83892 PIM2 antibody [9A5].
Loading : 5 ug per lane

Anti-PIM2 antibody [9A5] used in Flow cytometry (FACS). GTX83892

GTX83892 FACS Image

FACS analysis of HeLa cells using GTX83892 PIM2 antibody [9A5].
Red : Primary antibody
Blue : Negative control antibody

REFERENCE

Application Reference

Yang T et al. Oncogene 2018; (Epub) pim2-mediated phosphorylation of hexokinase 2 is critical for tumor growth and * resistance in breast cancer.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519