GeneTex

  • Country / Location Selection

United States (US)

PIM2 antibody

Anti-PIM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX113928
Anti-PIM2 antibody used in Western Blot (WB). GTX113928
Anti-PIM2 antibody used in Western Blot (WB). GTX113928

Cat. No. GTX113928

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, IHC

Reactivity

 Human, Mouse
Package
100 μl ($419),
25 μl ($169)
See all PIM2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

mouse testis , 293T

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PIM2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037694

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Pim-2 proto-oncogene, serine/threonine kinase

Background

PIM2 belongs to a family of protooncogenes that encode serine/threonine protein kinases (Mikkers et al., 2004 [PubMed 15199164]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PIM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX113928

GTX113928 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using PIM2(GTX113928) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PIM2 antibody used in Western Blot (WB). GTX113928

GTX113928 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX113928 diluted at 1:1000

Anti-PIM2 antibody used in Western Blot (WB). GTX113928

GTX113928 WB Image

PIM2 antibody detects PIM2 protein by Western blot analysis.
A. 50 μg mouse testis lysate/extract
12 % SDS-PAGE
PIM2 antibody (GTX113928) dilution: 1:500

REFERENCE

Application Reference

Sohni A et al. Cell Rep 2019; 26 (6) : 1501-1517.e4 The Neonatal and Adult Human Testis Defined at the Single-Cell Level.
Application : IHC
Reactivity : Human
Yu Z et al. J Biol Chem 2013; 288 (49) : 35406-16 Proviral insertion in murine lymphomas 2 (PIM2) oncogene phosphorylates pyruvate kinase M2 (PKM2) and promotes glycolysis in cancer cells.
Application : WB, ICC/IF, IP
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for PIM2 antibody (GTX113928). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169