APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human PIN1
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
peptidylprolyl cis/trans isomerase, NIMA-interacting 1 , DOD , UBL5
Cellular Localization
Nucleus,Nucleus speckle,Cytoplasm
Background
Peptidyl-prolyl cis/trans isomerases (PPIases) catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds. This gene encodes one of the PPIases, which specifically binds to phosphorylated ser/thr-pro motifs to catalytically regulate the post-phosphorylation conformation of its substrates. The conformational regulation catalyzed by this PPIase has a profound impact on key proteins involved in the regulation of cell growth, genotoxic and other stress responses, the immune response, induction and maintenance of pluripotency, germ cell development, neuronal differentiation, and survival. This enzyme also plays a key role in the pathogenesis of Alzheimer's disease and many cancers. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]
Database
Research Area
DATA IMAGES
![Anti-PIN1 antibody [HL2115] used in Western Blot (WB). GTX638087](/templates/default/js/lightbox/images/loading.gif "Anti-PIN1 antibody [HL2115] used in Western Blot (WB). GTX638087")
|
GTX638087 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PIN1 antibody [HL2115] (GTX638087) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
|
|
|
GTX638087 WB Image
Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PIN1 antibody [HL2115] (GTX638087) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX638087 ICC/IF Image
PIN1 antibody [HL2115] detects PIN1 protein at cytoplasm by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PIN1 stained by PIN1 antibody [HL2115] (GTX638087) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
|
|
|
GTX638087 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PIN1 antibody [HL2115] (GTX638087) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
REFERENCE
There are currently no references for PIN1 antibody [HL2115] (GTX638087). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PIN1 antibody [HL2115] (GTX638087). Be the first to share your experience with this product.
![Anti-PIN1 antibody [HL2115] used in Western Blot (WB). GTX638087](/upload/website/prouct_img/small/GTX638087/GTX638087_T-44907_20221230_WB_23010400_538.webp "Anti-PIN1 antibody [HL2115] used in Western Blot (WB). GTX638087")
![Anti-PIN1 antibody [HL2115] used in Western Blot (WB). GTX638087](/upload/website/prouct_img/small/GTX638087/GTX638087_T-44907_20221230_WB_M_R_23010400_853.webp "Anti-PIN1 antibody [HL2115] used in Western Blot (WB). GTX638087")
![Anti-PIN1 antibody [HL2115] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638087](/upload/website/prouct_img/small/GTX638087/GTX638087_T-44907_20230519_ICC_IF_R_23053001_691.webp "Anti-PIN1 antibody [HL2115] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638087")
![Anti-PIN1 antibody [HL2115] used in Western Blot (WB). GTX638087](/upload/website/prouct_img/small/GTX638087/GTX638087_44970_20230421_WB_M_R_23042500_348.webp "Anti-PIN1 antibody [HL2115] used in Western Blot (WB). GTX638087")
![Anti-PIN1 antibody [HL2115] used in Western Blot (WB). GTX638087](/upload/website/prouct_img/normal/GTX638087/GTX638087_T-44907_20221230_WB_23010400_538.webp "Anti-PIN1 antibody [HL2115] used in Western Blot (WB). GTX638087")
![Anti-PIN1 antibody [HL2115] used in Western Blot (WB). GTX638087](/upload/website/prouct_img/normal/GTX638087/GTX638087_T-44907_20221230_WB_M_R_23010400_853.webp "Anti-PIN1 antibody [HL2115] used in Western Blot (WB). GTX638087")
![Anti-PIN1 antibody [HL2115] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638087](/upload/website/prouct_img/normal/GTX638087/GTX638087_T-44907_20230519_ICC_IF_R_23053001_691.webp "Anti-PIN1 antibody [HL2115] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638087")
![Anti-PIN1 antibody [HL2115] used in Western Blot (WB). GTX638087](/upload/website/prouct_img/normal/GTX638087/GTX638087_44970_20230421_WB_M_R_23042500_348.webp "Anti-PIN1 antibody [HL2115] used in Western Blot (WB). GTX638087")