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PIN1 antibody [N1C3-2]

Anti-PIN1 antibody [N1C3-2] used in Western Blot (WB). GTX113245
Anti-PIN1 antibody [N1C3-2] used in Western Blot (WB). GTX113245
Anti-PIN1 antibody [N1C3-2] used in Western Blot (WB). GTX113245
Anti-PIN1 antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113245

Cat. No. GTX113245

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PIN1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

HepG2 , Raji , PC-12 , Rat2 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12

Predict Reactivity

Bovine, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PIN1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037695

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peptidylprolyl cis/trans isomerase, NIMA-interacting 1 , DOD , UBL5

Cellular Localization

Nucleus

Background

The human PIN1 gene encodes an essential nuclear peptidylprolyl cis-trans isomerase (PPIase; EC 5.2.1.8) involved in regulation of mitosis. PIN1 belongs to a class of PPIases that includes the E. coli parvulin, yeast Ess1, and Drosophila dodo (dod) gene products (Lu et al., 1996 [PubMed 8606777]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PIN1 antibody [N1C3-2] used in Western Blot (WB). GTX113245

GTX113245 WB Image

PIN1 antibody detects PIN1 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
15 % SDS-PAGE
PIN1 antibody (GTX113245) dilution: 1:1000

Anti-PIN1 antibody [N1C3-2] used in Western Blot (WB). GTX113245

GTX113245 WB Image

PIN1 antibody detects PIN1 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
15 % SDS-PAGE
PIN1 antibody (GTX113245) dilution: 1:1000

Anti-PIN1 antibody [N1C3-2] used in Western Blot (WB). GTX113245

GTX113245 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
B: Raji
12% SDS PAGE
GTX113245 diluted at 1:1000

Anti-PIN1 antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113245

GTX113245 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using PIN1(GTX113245) antibody at 1:200 dilution.

REFERENCE

Application Reference

Han YC et al. Sci Rep 2023; 13 (1) : 11088 VDR alleviates endothelial cell injury in arteriovenous fistula through inhibition of P66Shc-mediated mitochondrial ROS.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169