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PINK1 antibody [N3C3]

Anti-PINK1 antibody [N3C3] used in Western Blot (WB). GTX107851
Anti-PINK1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107851
Anti-PINK1 antibody [N3C3] used in Western Blot (WB). GTX107851

Cat. No. GTX107851

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PINK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951246

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PTEN induced kinase 1 , BRPK , PARK6

Cellular Localization

Mitochondrion outer membrane; Single-pass membrane protein

Background

This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PINK1 antibody [N3C3] used in Western Blot (WB). GTX107851

GTX107851 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
7.5% SDS PAGE
GTX107851 diluted at 1:1000

Anti-PINK1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107851

GTX107851 ICC/IF Image

PINK1 antibody [N3C3] detects PINK1 protein at mitochondria by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: PINK1 protein stained by PINK1 antibody [N3C3] (GTX107851) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-PINK1 antibody [N3C3] used in Western Blot (WB). GTX107851

GTX107851 WB Image

The data was published in the 2020 in Int J Mol Sci. PMID: 33260618
The data was published in the 2020 in Int J Mol Sci. PMID: 33260618

REFERENCE

REVIEW

PINK1 antibody [N3C3] Cat. No. GTX107851

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2020 in Int J Mol Sci. PMID: 33260618
Date : Anonymous submitted on 31-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : Hepa 1-6 cells
Amount used : 20 μg
Blocking : 5% skim milk in TBST 0.1% Tween-20, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169
Currently in production