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PINK1 antibody [N3C3]

Anti-PINK1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107851
Anti-PINK1 antibody [N3C3] used in Western Blot (WB). GTX107851

Cat No. GTX107851

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PINK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951246

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

PTEN induced kinase 1 , BRPK , PARK6

Cellular Localization

Mitochondrion outer membrane; Single-pass membrane protein

Background

This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PINK1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107851

GTX107851 ICC/IF Image

PINK1 antibody [N3C3] detects PINK1 protein at mitochondria by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: PINK1 protein stained by PINK1 antibody [N3C3] (GTX107851) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-PINK1 antibody [N3C3] used in Western Blot (WB). GTX107851

GTX107851 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
7.5% SDS PAGE
GTX107851 diluted at 1:1000

REFERENCE

REVIEW

PINK1 antibody [N3C3] Cat No. GTX107851

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 31-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : Hepa 1-6 cells
Amount used : 20 μg
Blocking : 5% skim milk in TBST 0.1% Tween-20, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169