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PINK1 antibody [N3C3]

Orthogonal
Anti-PINK1 antibody [N3C3] used in Western Blot (WB). GTX107851
Anti-PINK1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107851
Anti-PINK1 antibody [N3C3] used in Western Blot (WB). GTX107851

Cat. No. GTX107851

Reference 10
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PINK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PINK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951246

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PTEN induced kinase 1 , BRPK , PARK6

Cellular Localization

Mitochondrion outer membrane; Single-pass membrane protein

Background

This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PINK1 antibody [N3C3] used in Western Blot (WB). GTX107851

GTX107851 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
7.5% SDS PAGE
GTX107851 diluted at 1:1000

Anti-PINK1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107851

GTX107851 ICC/IF Image

PINK1 antibody [N3C3] detects PINK1 protein at mitochondria by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: PINK1 protein stained by PINK1 antibody [N3C3] (GTX107851) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-PINK1 antibody [N3C3] used in Western Blot (WB). GTX107851

GTX107851 WB Image

Orthogonal

The data was published in the 2020 in Int J Mol Sci. PMID: 33260618

REFERENCE

Application Reference

F Rostamzadeh et al. Biomed Pharmacother 2023; 170 : 116020 Therapeutic effects of the combination of moderate-intensity endurance training and MitoQ supplementation in rats with isoproterenol-induced myocardial injury: The role of mitochondrial fusion, fission, and mitophagy
Liao Y et al. BMC Cardiovasc Disord 2023; 23 (1) : 582 Abnormalities in the SIRT1-SIRT3 axis promote myocardial ischemia-reperfusion injury through ferroptosis caused by silencing the PINK1/Parkin signaling pathway.
Zhewen Zhang et al. Curr Issues Mol Biol 2022; 44 (6) : 2759-2771 Parkin, as a Regulator, Participates in Arsenic Trioxide-Triggered Mitophagy in HeLa Cells.
Application : WB
Reactivity : Human
Beak JY et al. J Biol Chem 2021; 297 (6) : 101358 The nuclear receptor RORα preserves cardiomyocyte mitochondrial function by regulating caveolin-3-mediated mitophagy.
Application : WB
Reactivity : Rat
Chen CY et al. Sci Rep 2021; 11 (1) : 2377 Cardiac-specific microRNA-125b deficiency induces perinatal death and cardiac hypertrophy.
Application : WB
Reactivity : Mouse
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Submit a Reference

REVIEW

PINK1 antibody [N3C3] Cat. No. GTX107851

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-PINK1 antibody [N3C3] used in Western Blot (WB). GTX107851
Date : Anonymous submitted on 31-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : Hepa 1-6 cells
Amount used : 20 μg
Blocking : 5% skim milk in TBST 0.1% Tween-20, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
F Rostamzadeh et al. Biomed Pharmacother 2023; 170:116020 Therapeutic effects of the combination of moderate-intensity endurance training and MitoQ supplementation in rats with isoproterenol-induced myocardial injury: The role of mitochondrial fusion, fission, and mitophagy
Liao Y et al. BMC Cardiovasc Disord 2023; 23 (1):582 Abnormalities in the SIRT1-SIRT3 axis promote myocardial ischemia-reperfusion injury through ferroptosis caused by silencing the PINK1/Parkin signaling pathway.
Zhewen Zhang et al. Curr Issues Mol Biol 2022; 44 (6):2759-2771 Parkin, as a Regulator, Participates in Arsenic Trioxide-Triggered Mitophagy in HeLa Cells.
Application : WB
Reactivity : Human
Beak JY et al. J Biol Chem 2021; 297 (6):101358 The nuclear receptor RORα preserves cardiomyocyte mitochondrial function by regulating caveolin-3-mediated mitophagy.
Application : WB
Reactivity : Rat
Rey F et al. Antioxidants (Basel) 2021; 10 (1) Mitochondrial Metabolism as Target of the Neuroprotective Role of Erythropoietin in Parkinson's Disease.
Application : WB
Reactivity : Human
Chen CY et al. Sci Rep 2021; 11 (1):2377 Cardiac-specific microRNA-125b deficiency induces perinatal death and cardiac hypertrophy.
Application : WB
Reactivity : Mouse
Haque MN et al. Phytomedicine 2021; 81:153415 The potential LXRβ agonist stigmasterol protects against hypoxia/reoxygenation injury by modulating mitophagy in primary hippocampal neurons.
Application : WB
Reactivity : Rat
Martina Moras et al. Int J Mol Sci 2020; 21 (23) Downregulation of Mitochondrial TSPO Inhibits Mitophagy and Reduces Enucleation during Human Terminal Erythropoiesis.
Application : WB
Reactivity : Human
Liu X et al. PLoS One 2017; 12 (8):e0182558 Astragaloside IV ameliorates diabetic nephropathy by modulating the mitochondrial quality control network.
Application : WB
Shi J et al. Cell Death Dis 2015; 6:e1943 NBR1 is dispensable for PARK2-mediated mitophagy regardless of the presence or absence of SQSTM1.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169
Currently in production