GeneTex
United States (US)

PION antibody

Anti-PION antibody used in Western Blot (WB). GTX32001
Anti-PION antibody used in IHC (Paraffin sections) (IHC-P). GTX32001
Anti-PION antibody used in IHC (Paraffin sections) (IHC-P). GTX32001
Anti-PION antibody used in IHC (Paraffin sections) (IHC-P). GTX32001

Cat No. GTX32001

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.25 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

98 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , EL4 Cell Lysate(GTX27183)

Specificity/Sensitivity

Multiple isoforms of PION are known to exist. PION antibody is predicted to not cross-react with other F-box protein family members.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PION antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human PION.The immunogen is located within amino acids 770 - 820 of PION.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Gamma-Secretase Activating Protein,Pion,Gsap

Background

Accumulation of neurotoxic amyloid-beta is a major hallmark of Alzheimer disease (AD; MIM 104300). Formation of amyloid-beta is catalyzed by gamma-secretase (see PSEN1; MIM 104311), a protease with numerous substrates. PION, or GSAP, selectively increases amyloid-beta production through a mechanism involving its interaction with both gamma-secretase and its substrate, the amyloid-beta precursor protein (APP; MIM 104760) C-terminal fragment (APP-CTF) (He et al., 2010 [PubMed 20811458]).[supplied by OMIM, Nov 2010]

Database

Research Area

DATA IMAGES
Anti-PION antibody used in Western Blot (WB). GTX32001

GTX32001 WB Image

WB analysis of EL4 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX32001 PION antibody.
Working concentration : 0.25 μg/ml

Anti-PION antibody used in IHC (Paraffin sections) (IHC-P). GTX32001

GTX32001 IHC-P Image

IHC-P analysis of human brain tissue using GTX32001 PION antibody.
Working concentration : 20 μg/ml

Anti-PION antibody used in IHC (Paraffin sections) (IHC-P). GTX32001

GTX32001 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX32001 PION antibody.
Working concentration : 5 μg/ml

Anti-PION antibody used in IHC (Paraffin sections) (IHC-P). GTX32001

GTX32001 IHC-P Image

IHC-P analysis of human brain tissue using GTX32001 PION antibody.
Working concentration : 5 μg/ml

Package List Price ($)
$ 329