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PIP4K2B antibody [N1C1]

Anti-PIP4K2B antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX111751
Anti-PIP4K2B antibody [N1C1] used in Western Blot (WB). GTX111751
Anti-PIP4K2B antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX111751

Cat. No. GTX111751

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PIP4K2B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PIP4K2B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951248

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphatidylinositol-5-phosphate 4-kinase type 2 beta , PI5P4KB , PIP5K2B , PIP5KIIB , PIP5KIIbeta , PIP5P4KB

Background

The protein encoded by this gene catalyzes the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. The encoded protein sequence does not show similarity to other kinases, but the protein does exhibit kinase activity. Additionally, the encoded protein interacts with p55 TNF receptor. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PIP4K2B antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX111751

GTX111751 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using PIPK I beta(GTX111751) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PIP4K2B antibody [N1C1] used in Western Blot (WB). GTX111751

GTX111751 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PIP4K2B antibody [N1C1] (GTX111751) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PIP4K2B antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX111751

GTX111751 IHC-P Image

PIP4K2B antibody [N1C1] detects PIP4K2B protein at cytoplasm in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
PIP4K2B antibody [N1C1] (GTX111751) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169