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PIP5K1A antibody [HL2011]

Anti-PIP5K1A antibody [HL2011] used in Western Blot (WB). GTX637912
Anti-PIP5K1A antibody [HL2011] used in Western Blot (WB). GTX637912
Anti-PIP5K1A antibody [HL2011] used in Western Blot (WB). GTX637912
Anti-PIP5K1A antibody [HL2011] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637912
Anti-PIP5K1A antibody [HL2011] used in Western Blot (WB). GTX637912
Anti-PIP5K1A antibody [HL2011] used in IHC (Paraffin sections) (IHC-P). GTX637912

Cat. No. GTX637912

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2011

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Product Note

This antibody is specific for human PIP5K1A protein, and it does not cross react with human PIP5K1B and PIP5K1C protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PIP5K1A. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphatidylinositol-4-phosphate 5-kinase type 1 alpha

Cellular Localization

Cell membrane,Cytoplasm,Nucleus speckle,Cell projection, lamellipodium,Nucleus

Background

Enables 1-phosphatidylinositol-4-phosphate 5-kinase activity and kinase binding activity. Involved in several processes, including activation of GTPase activity; focal adhesion assembly; and ruffle assembly. Located in several cellular components, including lamellipodium; nuclear speck; and ruffle membrane. Colocalizes with mRNA cleavage and polyadenylation specificity factor complex. [provided by Alliance of Genome Resources, Apr 2022]

Database

Research Area

DATA IMAGES

Anti-PIP5K1A antibody [HL2011] used in Western Blot (WB). GTX637912

GTX637912 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PIP5K1A antibody [HL2011] (GTX637912) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PIP5K1A antibody [HL2011] used in Western Blot (WB). GTX637912

GTX637912 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PIP5K1A antibody [HL2011] (GTX637912) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-PIP5K1A antibody [HL2011] used in Western Blot (WB). GTX637912

GTX637912 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with PIP5K1A antibody [HL2011] (GTX637912) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PIP5K1A antibody [HL2011] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637912

GTX637912 ICC/IF Image

PIP5K1A antibody [HL2011] detects PIP5K1A protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PIP5K1A stained by PIP5K1A antibody [HL2011] (GTX637912) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PIP5K1A antibody [HL2011] used in Western Blot (WB). GTX637912

GTX637912 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PIP5K1A antibody [HL2011] (GTX637912) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PIP5K1A antibody [HL2011] used in IHC (Paraffin sections) (IHC-P). GTX637912

GTX637912 IHC-P Image

PIP5K1A antibody [HL2011] detects PIP5K1A protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat spleen.
PIP5K1A stained by PIP5K1A antibody [HL2011] (GTX637912) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for PIP5K1A antibody [HL2011] (GTX637912). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations