APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody is specific for human PIP5K1A protein, and it does not cross react with human PIP5K1B and PIP5K1C protein.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human PIP5K1A. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
phosphatidylinositol-4-phosphate 5-kinase type 1 alpha
Cellular Localization
Cell membrane,Cytoplasm,Nucleus speckle,Cell projection, lamellipodium,Nucleus
Background
Enables 1-phosphatidylinositol-4-phosphate 5-kinase activity and kinase binding activity. Involved in several processes, including activation of GTPase activity; focal adhesion assembly; and ruffle assembly. Located in several cellular components, including lamellipodium; nuclear speck; and ruffle membrane. Colocalizes with mRNA cleavage and polyadenylation specificity factor complex. [provided by Alliance of Genome Resources, Apr 2022]
Database
Research Area
DATA IMAGES
![Anti-PIP5K1A antibody [HL2011] used in IHC (Paraffin sections) (IHC-P). GTX637912](/templates/default/js/lightbox/images/loading.gif "Anti-PIP5K1A antibody [HL2011] used in IHC (Paraffin sections) (IHC-P). GTX637912")
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GTX637912 IHC-P Image
PIP5K1A antibody [HL2011] detects PIP5K1A protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded cat spleen.PIP5K1A stained by PIP5K1A antibody [HL2011] (GTX637912) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637912 ICC/IF Image
PIP5K1A antibody [HL2011] detects PIP5K1A protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PIP5K1A stained by PIP5K1A antibody [HL2011] (GTX637912) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637912 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PIP5K1A antibody [HL2011] (GTX637912) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637912 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PIP5K1A antibody [HL2011] (GTX637912) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637912 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PIP5K1A antibody [HL2011] (GTX637912) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX637912 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with PIP5K1A antibody [HL2011] (GTX637912) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for PIP5K1A antibody [HL2011] (GTX637912). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PIP5K1A antibody [HL2011] (GTX637912). Be the first to share your experience with this product.
![Anti-PIP5K1A antibody [HL2011] used in IHC (Paraffin sections) (IHC-P). GTX637912](/upload/website/prouct_img/small/GTX637912/GTX637912_44935_20230714_IHC-P_Cat_23081619_505.webp "Anti-PIP5K1A antibody [HL2011] used in IHC (Paraffin sections) (IHC-P). GTX637912")
![Anti-PIP5K1A antibody [HL2011] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637912](/upload/website/prouct_img/small/GTX637912/GTX637912_T-44872_20230119_ICC_IF_23021401_792.webp "Anti-PIP5K1A antibody [HL2011] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637912")
![Anti-PIP5K1A antibody [HL2011] used in Western Blot (WB). GTX637912](/upload/website/prouct_img/small/GTX637912/GTX637912_44935_20230616_WB_D_23062019_987.webp "Anti-PIP5K1A antibody [HL2011] used in Western Blot (WB). GTX637912")
![Anti-PIP5K1A antibody [HL2011] used in Western Blot (WB). GTX637912](/upload/website/prouct_img/small/GTX637912/GTX637912_T-44872_20221209_WB_M_R_22121123_188.webp "Anti-PIP5K1A antibody [HL2011] used in Western Blot (WB). GTX637912")
![Anti-PIP5K1A antibody [HL2011] used in Western Blot (WB). GTX637912](/upload/website/prouct_img/small/GTX637912/GTX637912_T-44872_20221209_WB_22121123_362.webp "Anti-PIP5K1A antibody [HL2011] used in Western Blot (WB). GTX637912")
![Anti-PIP5K1A antibody [HL2011] used in Western Blot (WB). GTX637912](/upload/website/prouct_img/small/GTX637912/GTX637912_T-44872_20221216_WB_multiple_B_22122018_356.webp "Anti-PIP5K1A antibody [HL2011] used in Western Blot (WB). GTX637912")
![Anti-PIP5K1A antibody [HL2011] used in IHC (Paraffin sections) (IHC-P). GTX637912](/upload/website/prouct_img/normal/GTX637912/GTX637912_44935_20230714_IHC-P_Cat_23081619_505.webp "Anti-PIP5K1A antibody [HL2011] used in IHC (Paraffin sections) (IHC-P). GTX637912")
![Anti-PIP5K1A antibody [HL2011] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637912](/upload/website/prouct_img/normal/GTX637912/GTX637912_T-44872_20230119_ICC_IF_23021401_792.webp "Anti-PIP5K1A antibody [HL2011] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637912")
![Anti-PIP5K1A antibody [HL2011] used in Western Blot (WB). GTX637912](/upload/website/prouct_img/normal/GTX637912/GTX637912_44935_20230616_WB_D_23062019_987.webp "Anti-PIP5K1A antibody [HL2011] used in Western Blot (WB). GTX637912")
![Anti-PIP5K1A antibody [HL2011] used in Western Blot (WB). GTX637912](/upload/website/prouct_img/normal/GTX637912/GTX637912_T-44872_20221209_WB_M_R_22121123_188.webp "Anti-PIP5K1A antibody [HL2011] used in Western Blot (WB). GTX637912")
![Anti-PIP5K1A antibody [HL2011] used in Western Blot (WB). GTX637912](/upload/website/prouct_img/normal/GTX637912/GTX637912_T-44872_20221209_WB_22121123_362.webp "Anti-PIP5K1A antibody [HL2011] used in Western Blot (WB). GTX637912")
![Anti-PIP5K1A antibody [HL2011] used in Western Blot (WB). GTX637912](/upload/website/prouct_img/normal/GTX637912/GTX637912_T-44872_20221216_WB_multiple_B_22122018_356.webp "Anti-PIP5K1A antibody [HL2011] used in Western Blot (WB). GTX637912")