Wild-type (WT) and PIP5K1A knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PIP5K1A antibody [N1N3] (GTX111953) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed A549, using PIP5K1A(GTX111953) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using PIPK I alpha (GTX111953) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min