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PIP5K1A antibody [N1N3]

Anti-PIP5K1A antibody [N1N3] used in Western Blot (WB). GTX111953
Anti-PIP5K1A antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111953
Anti-PIP5K1A antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111953

Cat No. GTX111953

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Rat, Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PIP5K1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951250

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

phosphatidylinositol-4-phosphate 5-kinase type 1 alpha

Cellular Localization

Cell membrane , Endomembrane system , Golgi apparatus , Golgi stack

Database

Research Area

DATA IMAGES

Anti-PIP5K1A antibody [N1N3] used in Western Blot (WB). GTX111953

GTX111953 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PIP5K1A antibody [N1N3] (GTX111953) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PIP5K1A antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111953

GTX111953 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using PIPK I alpha (GTX111953) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PIP5K1A antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111953

GTX111953 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using PIP5K1A(GTX111953) antibody at 1:500 dilution.

REFERENCE

There are currently no references for PIP5K1A antibody [N1N3] (GTX111953). Be the first to share your publications with this product.

REVIEW

PIP5K1A antibody [N1N3] Cat No. GTX111953

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 19-May-2016
Score :
Application Tested : WB
Sample Species : Ms
Sample : RAW264.7 cells
Amount used : 30μg
Blocking : 5% skim milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:1000, 25°C, 1Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169