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PITRM1 antibody

Anti-PITRM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119739
Anti-PITRM1 antibody used in Western Blot (WB). GTX119739
Anti-PITRM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX119739

Cat. No. GTX119739

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PITRM1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

117 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Mouse, Rat, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PITRM1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617717

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pitrilysin metallopeptidase 1 , MP1 , PreP

Cellular Localization

Mitochondrion matrix

Database

Research Area

DATA IMAGES

Anti-PITRM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119739

GTX119739 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PITRM1(GTX119739) antibody at 1:200 dilution.

Anti-PITRM1 antibody used in Western Blot (WB). GTX119739

GTX119739 WB Image

Sample (30 ug of whole cell lysate)
A: A549
5% SDS PAGE
GTX119739 diluted at 1:1000

Anti-PITRM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX119739

GTX119739 IHC-P Image

PITRM1 antibody detects PITRM1 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: PITRM1 antibody (GTX119739) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169